SpectraMax 190Microplate Reader

A microplate reader for exploring the mysteries of life

Quantify DNA and perform endpoint, kinetic, and spectral scanning analyses with the UV-Vis SpectraMax 190 Microplate Reader. The multichannel design for 96-well microplates uses separate sample and reference beams for each sample — just like a dual-beam spectrophotometer — to eliminate error due to variations in optical fiber light output. Monochromator-based wavelength selection from 190 nm to 850 nm keeps you on the road to discovery, instead of tracking down and changing out filters.

  • Overview
  • Applications
  • Software
  • Specifications
  • Resources
The patented multichannel optical design of the SpectraMax 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting.

Benefits

  • Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm
  • Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaks
  • Measurement accuracy is increased due to narrow bandwidths that deliver increased and linearity over the widest range of assays, including DNA quantitation in the UV range
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy
  • Quickly quantify DNA, RNA, and protein samples as low as 2 µL with the SpectraDrop Micro-Volume Microplate

Features

  • Ability to monitor temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices’ own integrated microplate stacker designed exclusively for the SpectraMax Reader

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:
  • Comprehensive validation toolset available include:
  • SpectraTest ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
  • SoftMax Pro Software Validation Package
  • Software tools for FDA 21 CFR Part 11 compliance
The SpectraMax 190 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Micro volume applications
  • Microbial growth
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays

SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature




SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment’s needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience
  • Contemporary user community enabling protocol exchange and expertise sharing among our numerous users


  • Proven integration with leading automation and LIMS platforms for partnered solutions
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software

* available in select instrument models

General Specifications
Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13.6 kg)
Power Source 90-250 VAC, 50/60 Hz
Performance Specifications
Wavelength Range 190-850 nm
Wavelength Selection Monochromator, tunable 1/0 nm increments
Wavelength Bandwidth 2.0 nm
Wavelength Accuracy ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric  Resolution 0.001 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 – 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 – 2.0 OD
Stray Light ≤ 0.07% at 340 nm
OD ≥ 3.15
Light Source Xenon flash lamp
Microplate Read Time – Endpoint 9 seconds
Microplate Read Time – Kinetic 9 second minimum interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C