The digitizer that enables acquisition of patch-clamp data

The Axon™ Digidata® 1550B plus HumSilencer® is one of the most advanced analog-to-digital signal converters on the market, offering the innovative line synchronous noise-cancelling feature, HumSilencer. This advanced feature learns, adapts, and eliminates local 50/60 Hz line-frequency noise and high-frequency harmonics from incoming signals. The digitizer works with pCLAMPTM 11 Software for data acquisition and analysis. The software supports eight channels of analog acquisition and four acquisition modes.
  • Eliminate filters

    Eliminate line-frequency noise

    HumSilencer removes 50/60 hz line-frequency noise on up to four channels in parallel. The function is quickly enabled through a software checkbox.
  • Get consistent results

    Record high resolution traces

    All eight analog input channels can be simultaneously digitized at an impressive sampling rate of 500 kHz per channel.
  • Integrate with ease

    Digitize cellular network studies

    With Digidata 1550B system, you can now record cellular networks without 50/60 Hz line-frequency noise in a single digitizer while saving money, time, and reducing hassle.
SpectraMax ABS and ABS Plus Absorbance Microplate Readers
Introducing the Axon Digidata 1550B Low Noise Data Acquisition System plus HumSilencer
お見積り・お問い合わせ

Features

  • 50/60 hz noise cancellation

    HumSilencer is an advanced feature that learns, adapts, and eliminates local 50/60 Hz line-frequency noise patterns and associated high-frequency harmonics from incoming signal up to 20 V peak-to-peak.

  • Noise canceling

    HumSilencer is not a filter and has no effect on acquired signals. It causes no signal distortion such as frequency change, amplitude attenuation, phase shift, or DC voltage change.

  • Wide range of input signals

    Digidata 1550B digitizes a wide range of input signals from -10 to +10 V.

  • HumSilencer adaptation

    HumSilencer automatically recognizes changes in noise amplitudes and adapts by removing noise in less than one second.

  • Optimized for pCLAMP 11 Software

    The pCLAMP Software suite is the most widely-used patch-clamp electrophysiology data acquisition and analysis program for control and recording of voltage-clamp, current-clamp, and patch-clamp experiments.

  • Three configurations

    Three configurations are available: Digidata 1550B4 with four HumSilencer channels, Digidata 1550B1 with one HumSilencer channel, and Digidata 1550B0 without a HumSilencer channel.
View all Resources

Applications of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

  • Digital Acquisition

    The current or voltage signal acquired by the amplifier is an analog signal, but to perform data analysis needed for high resolution patch-clamp measurements, the analog signal must be converted into a digital one. Positioned between the amplifier and the computer, the digitizer accomplishes this important task. Signal quality is extremely important and is impacted by the sampling frequency. The latest generation of Digidata digitizers sample at 500 kHz and can be equipped with HumSilencer, which eliminates 50/60 Hz line-frequency noise.

  • HumSilencer Technology

    50/60 Hz line-frequency noise, also known as electrical hum, is the most common source of background noise in patch-clamp electrophysiology experiments. This noise can overwhelm biological signals of interest, making sensitive patch-clamp measurements nearly impossible. Traditional trouble shooting is typically only partially effective and can impair data accuracy. HumSilencer is a filter-free, adaptive technology that learns and removes line frequency noise without using methods that impair signal accuracy like filters, that can distort biological signals.

  • Cellular Pathway Analysis

    Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, and neuronal communications.

  • Disease Research

    Ion channels play a role in many diseases including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain, and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease.

Specifications & Options of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Request a Quote
General specifications
Maximum input signal (total of noise + signal)

±10 V

Maximum noise amplitude

20 V, peak-to-peak (on a 0 V signal)

Noise cancellation

Line-frequency (50 Hz / 60 Hz) and harmonics to 10 kHz

Cancellation response time

< 1 second
Number of input channels

8 single-ended

Number of ADCs

8

Sampling rates

1 Hz – 500 kHz

Input range

-10.000 V to +10.000 V

Input resistance

>1 MΩ

Gain value

1

Number of channels

8

Number of DAC

8

Output range

-10.000 V to +10.000 V

Output impedance

< 0.5 Ω

Output short circuit to signal ground

±25 mA

Number of bits

8 (of 16) supported in software

SCOPE

Dedicated trigger output

Output driver

Advanced CMOS (AC) compatible

Output current

±4 mA source, cable

Type

USB 2.0 braided

Length

3 meters

Input type

TTL compatible

TAG

Rising-edge sensitive

Resolution

16-bit

Resources of Axon Digidata 1550B Low-Noise Data Acquisition System plus HumSilencer

Videos and Demos

Calculate Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
Introducing the Axon Digidata 1550B Low Noise Data Acquisition System plus HumSilencer
Calculate Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
How to Combine Traces, Calculate Rise or Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
Create Customized Command Waveforms Using pCLAMP Software
How to Create Customized Command Waveforms Using the pCLAMP Software
Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software
The Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software
Synchronizing Electrophysiology and Imaging Solutions with pCLAMP and MetaMorph Software
Synchronizing Electrophysiology and Imaging Solution with Axon pCLAMP and MetaMorph Software
Membrane Test Between Sweeps in Clampex & Analysis of Synaptic Events with Clampfit Data Analysis
Online Statistics, Membrane Test Between Sweeps in Clampex and Analysis of Synaptic Events with the Clampfit™ Data Analysis
Use of Axoporator for Single-cell Electroporation for Transfection and Dye-labeling
Use of the Axoporator 800A for Single-cell Electroporation for Transfection and Dye-labeling
Using Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels
Using the Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels
Use of Filters in Data Acquisition and the Clampfit Application
Writing Long-Term Potentiation and Depression Protocols and the Use of Filters in Data Acquisition and the Clampfit Application
Resistance Compensated Series
Series Resistance Compensated or Not
Using Electrophysiological Studies to Accelerate Mechanistic in Reception and Transmission
Using Electrophysiological Studies to Accelerate Mechanistic Study in Reception and Transmission
Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning
Update and Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning
Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in Xenopus Oocyte Model
Investigations of the Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in a Xenopus Oocyte Model
Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies
Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies
Axon Amplifiers and pCLAMP Software-key Features Reviews (Chinese version)
Axon Amplifiers and pCLAMP Software-key Features Reviews (Chinese version)
Using Clampfit in Basic Single Channel Analysis
Basic Single Channel Analysis Using Clampfit
Action Potential Analysis in Clampfit Module
Action Potential Analysis in Clampfit Module
A Walkthrough of Protocol Editor in pCLAMP Data Acquisition Module
A Walkthrough of the Protocol Editor in the pCLAMP Data Acquisition Module
A Walkthrough of Protocol Editor in pCLAMP (Chinese version)
A Walkthrough of Protocol Editor in pCLAMP (Chinese version)

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.

お見積り・お問い合わせ