ImageXpress Micro 4ハイコンテント イメージングシステム

Screening

• Determine the mechanisms of action for the research of cancer, neurological disorders, viral infection, and other diseases with compound or RNAi screening
• Quantitate multiplexed cellular responses, such as apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, and cell signaling
• Detect one to four fluorescent markers along with the ability to resolve their colocalization

Unstimulated transfluor-expressing cells wtih cytosolic distribution of beta arrestin GFP. Nuclei stained in blue, mitochondria in red.

Stimulated transfluor-expressing cells with beta-arrestin GFP that has redistributed to vesicles. Nuclei stained in blue, Mitochondria in red.

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for cell health, cell cycle, micronuclei, neurite outgrowth, angiogenesis, translocation, multi-wavelength cell scoring and granularity.

RNA Interference

• Interrogate large-scale RNAi libraries
• Measure critical events including apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, cell signaling, and multi-wavelength cell scoring.
• Automatically identify, segment, and analyze biological phenotypes using validated software modules

Images of control cells showing DNA damage detected by H2AX phosphorylation. The positive control (bottom) clearly shows a higher percentage of cells with DNA damage (green) compared to total cells (red) in the well.

Further Information

• Watch Recorded Webinars:
  • Using High-Throughput RNAi Screening to Identify Cellular Factors Involved in Host-Pathogen Interactions
  • Preparing Assays for Genome-wide RNAi Screening Using High Content Microscopy

Stem Cell Research

• Measure stem cell growth and differentiation in layers or colonies with the system’s widefield focusing capabilities, brightfield imaging option, and environmental control chamber for extended period of time
• Accurately track cell processes, colony size or number across multiple fields of view with unique stitching algorithm and precise plate stage movements
• Gain insight into the complex molecular and genetic signaling events that underlie cell proliferation and differentiation
• Accurately measure nuronal process across multiple fields of view using unique stitching algorithm with precise stage movements
• Analyze 3D samples by capturing an image stack in the Z-plane within each field of view and collapse it into a best-focus image or view the sample in 3D

Differentiated neurons imaged in a 96-well plate at 10x magnification with three different fluorescent markers

Further Information

• Download Application Notes:
  • Multiplexed High Content Hepatotoxicity Assays Using iPSC-Derived Hepatocytes
  • High Content Screening of Neuronal Toxicity Using iPSC-Derived Human Neurons
  • ImageXpress Micro System as a Solution for Monitoring Neuronal Stem Cell Expansion and Differentiation

• Watch Recorded Webinar:
  • Contemporary Automation and High content Imaging Tools for Screening Stem Cell-Derived Cardiomyocytes

• Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth, translocation, cell scoring and multi wavelength cell scoring.

Live Cells

• Monitor cell growth, death, differentiation, and migration; viral or bacterial invasion, cancer metastasis, chemotaxis, drug toxicity, or translocation
• Observe rapid kinetic cellular responses over a span of several days using fluorescent or unlabeled cells kept in an environmental control chamber, together with a brightfield microscope, and on-board fluid addition capabilities

Watch a 30-hour time-course movie of increasing apoptotic and dead HeLa cells after treatment with 100 µm Etoposide. Blue = Hoechst for all nuclei; Red = Propidium Iodide for dead cells; Green = Nucview488, a fluorescence substrate for Caspase 3 and an apoptosis sensor. Overlaid on a phase contrast image..

• Watch Recorded Webinar:
  • Live Cell Imaging to Investigate the Regulation of Cell Division Timing

Whole Organism Imaging

• Image an entire well
• Keep organisms in focus for the duration of experiment
• Perform sophisticated analysis of images and create time-lapse videos

3D Imaging

• Monitor cell health kinetics under environmental control for hours or days
• Reconstruct 3D spheroids with rapid z-stack imaging
• Study spheroids with readily available plates and straightforward imaging workflow
• Acquire from a variety of multi-well plate formats up to 1536 including unusual formats developed for 3D assays including round bottom and trans-well plates

Further Information

Download Application Notes:

• High-throughput confocal imaging of spheroids for screening cancer therapeutics
• 3D Imaging of Cancer Spheroids

Watch Recorded Webinars:

• Setting up 3D Spheroid Assays
• 3D Imaging of Cancer Spheroids

Thick samples

• Image neurons or stem cells grown on 3D matrices that can make focusing on the cells of interest a challenge
• Capture difficult-to-image thick tissue sections (at least 20 µm thick) that produce high-background fluorescence when stained
• Analyze 3D samples by collecting a stack of crisp images in the Z-plane in as small as 100 nm increments

Neuronal processes can be measured and quantified with a Neurite Outgrowth Application Module in MetaXpress Software.

Mouse Intestine section stained for nuclei (green), filamentous actin prevalent in the brush border (red), goblet cells (blue)

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth and angiogenesis application modules.

Homogeneous Binding Assay

• Multiplex different cells in the same plate well
• Run assays with adherent cells, suspension cells, or beads
• Detect fluorescent labels at any wavelength

Click here to learn more about MetaXpress Application Modules that enable some common HCS assays.

Molecular Devices brings the image acquisition and analysis for ImageXpress systems under one common platform to streamline workflow, offering a truly end-to-end solution for high-content screening.

MetaXpress® High-Content Image Acquisition & Analysis Software

• MetaXpress Software Application Modules: turnkey solutions for your 100 most common analysis routines
• MetaXpress Software Custom Modules: flexible toolbox for advanced assays, including 3D structure analysis with volume, intensity, and distance measurements.
• MetaXpress Software Journals: powerful macros to analyze unique applications

The ImageXpress Micro 4 High-Content Imaging System offers researchers an agile imaging platform with field upgradeable confocal imaging. For more details on the confocal system, please click here.

Additionally users can configure the instrument to fit their specific research.

For more information on product configurations and pricing, please click here.

Widefield Light Source Options

Solid state white-light engine with long lifetime, suitable for most fluorescent applications

• Lifetime: 20,000 hrs. Wavelength: 380-680 nm
• Included in ImageXpress Micro Imaging systems

Broad spectrum long-life light source is suitable for most fluorescent applications, 10 position filter wheel included for ratio imaging (Fura-2; BCECF). Compatible with multi-pass excitation cubes for additional flexibility and speed.

• Lifetime: 10,000+ hrs. Wavelength: 340-680 nm
• Optional upgrade for ImageXpress Micro 4 system.
• Filter Wheel Features
  • 10 position excitation filter wheel increases the number of preconfigured filters installed with systems to support multi-pass dichroic and emission filters in core facilities with a large variety of fluorophores in use.
  • Fast filter exchange is ideal for exciting ratiometric indicators (e.g. Fura-2; BCECF) to monitor fast cellular responses and real-time kinetics indicative of ion exchange and intracellular signaling.
• Filter Wheel Specifications
  • Controller drives one filter wheel
  • Wheel can hold up to 10 excitation filters
  • Switching times of up to 40msec between adjacent filters
  • User adjustable speed allows the speed to load matching
  • Wheel is inserted into the light source so additional space is not required

Near-IR optimized solid state whitelight engine with long lifetime, suitable for most fluorescent applications enhanced for near-IR application like Cy7

• Lifetime: 10,000+ hrs. Wavelength: 380-770 nm
• Optional upgrade for ImageXpress Micro 4 system

Configurable solid state light engine is a customizable excitation source with independent control of up to 7 wavelengths. It expands flexibility of wavelength selection and is compatible with multi-pass excitation cubes for additional throughput and speed.

• Lifetime: 10,000+ hrs. Wavelength: Customizable 380 nm to NIR
• Optional upgrade for ImageXpress Micro 4 system

Transmitted Light

The ImageXpress Micro 4 High-Content Imaging System enables contrast imaging of unstained samples to monitor cells that may be overly sensitive to the effects of fluorescent labels or need to be monitored over time without intervention. The Phase Contrast option can be used to scrutinize cell quality before and after biochemical assays, or combined with fluorescent applications and environmental control to examine multiple parameters at once.

Features

• Phase optics enable high contrast, label-free cellular imaging to be performed at a variety of magnifications (4x -60x)
• Overlay fluorescent images on unstained images gives the user another fluorophore independent visualization of cell morphology

Specifications

• Nikon 100w Pillar Diascopic Illuminator with TE-C ELWD Condenser
• The condenser is 0.3NA with 65 mm WD and PhL, Ph1, and Ph2 selectable phase rings
• Phase Contrast option is compatible with Environmental Control, but not with Fluidics Option

Environmental Control

The Environmental Control Option for the ImageXpress Micro 4 High-Content Imaging System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high-content screening kinetic and time lapse experiments a reality.

Features

• User-defined premixed air allows researchers to select atmospheric conditions most appropriate for their cell type (e.g. 5% or 10% CO2).
• Temperature control of the sample plate helps maintain physiological environment.
• Humidity regulation is maintained above sample plate to minimize evaporation of media and extend measurement time.
• Our high resolution stage and environmental control enable fast kinetic imaging of live cells or long time lapse monitoring of each well in a plate for hours or days.

Specifications

• Accepts pressurized air from premixed tanks
• Temperature maintained in top half of instrument: 30-40°C ± 0.5°C
• Temperature measured near the sample plate is recorded and stored with each image in software
• Humidity maintained above plate; evaporation < 0.5 μL/well/hour (96- or 384-well formats)
• Compatible with Fluidics or Phase Contrast Transmitted Light options

Fluidics

During assay development, the Fluidics option facilitates compound addition and cell washing to characterize potency or to determine incubation times for higher throughput fixed time point assays.

Features

• Configurable pipettor can accommodate 96- or 384-well pipette tips, compound plates and sample plates giving you the flexibility to change assay formats when you wish
• Tip exchange option minimizes cross contamination often seen with non-contact, piezo- or pin tool-based fluidics systems
• Integrated pipettor (3-200 µL) facilitates media replenishment or compound addition to individual wells within a microplate. Media replenishment can extend the time frame of long time lapse experiments and overcome any evaporation that may occur over time
• Custom Light Source option can be combined with liquid handling to monitor dual-excitation wavelength intracellular ion concentration measurements (e.g. calcium, pH) to provide greater insight to ion exchange and intracellular function

Specifications

• Single-channel pipetting
• Dispenses volumes from 3 μL to 200 μL
• ±1 from 3 μL to 20 μL ±5% from 20 μL to 200 μL
• Holds two compound or media plates with optional heating
• 96- or 384-well format FLIPR® tips
• Comes standard with Environmental Control option
• Not compatible with Phase Contrast Transmitted Light option

Camera

4.66 megapixel scientific CMOS

Stages

Optically-encoded Liner voice coil driven stages with encoders that measure position with better than 25nm resolution to achieve high accuracy and precision in X, Y, and Z axes

Objectives

Variety of Nikon 1x- 100x air and oil immersion lenses are supported.

Standard Filter Options

Fluorophore	Part Number	Ex (nm)	Dichroic (nm)	EM (nm)
DAPI, Alexa Fluor 350, AMCA, BFP, Hoechst, LysoSensor Blue, Marina Blue, Pacific Blue, and sgBFP Obsolete August 2016	1-6300-0442	377/50	327-404 (R) 415-950 (T)	447/60
CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1	1-6300-0447	438/24	426-450 (R) 467-950 (T)	483/32
GFP, Cy2™, CyQuant, DiO, EGFP, sgGFP™, wtGFP (non-UV excitation), YOYO-1, YO-PRO-1	1-6300-0450	472/30	350-488 (R) 502-950 (T)	520/35
FITC, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP (red-shifted GFP, S65T)	1-6300-0443	482/35	350-500 (R) 513-950 (T)	536/40
YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123	1-6300-0448	500/24	350-512 (R) 528-950 (T)	542/27
TRITC, Alexa Fluor 546, 5-TAMRA, Alexa Fluor 532, SpectrumOrange™, BODIPY TMR, DiI, POPO-3, PO-PRO-3, Rhodamine	1-6300-0444	543/22	350-555 (R) 569-950 (T)	593/40
Cy3™, DsRed, Alexa Fluor 555, Calcium Orange, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin (PE), Rhodamine Phallicidine, and TAMRA	1-6300-0445	531/40	350-555 (R) 569-950 (T)	593/40
Texas Red®, mCherry, 5-ROX, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5™, HcRed, Propidium Iodide, and MitoTracker Red	1-6300-0449	562/40	350-585 (R) 601-950 (T)	624/40
Cy5™, SpectrumFRed™, Alexa Fluor 647 & 660, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, DRAQ5, TOTO-3, and TO-PRO-3	1-6300-0446	628/40	350-651 (R) 669-950(T)	692/40
FITC, FAM, Alexa, Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP (red-shift GFP, S65T)	5045173 (system shipped after August 2017)	474/27	350-488R 502-950T	525/45

Additional filters as well as custom configurations are available upon request. Filter specifications are current as of 9/1/2015.

Space And Weight Specifications

ImageXpress Micro 4 system configuration	W x D x H	Weight
ImageXpress Micro Base Unit System	21 x 34 x 19 (Inches) 53 x 86 x 48 (Centimeters)	215 (Lbs) 95 (Kg)
Transmitted Light and Live Cell (Environmental Control)	21 x 34 x 32 (Inches) 53 x 86 x 81 (Centimeters)	220 (Lbs) 100 (Kg)
Live Cell (Environmental Control) and Fluidics	21 x 34 x 42 (Inches) 53 x 86 x 107 (Centimeters)	295 (Lbs) 134 (Kg)
Transmitted Light	21 x 34 x 32 (Inches) 53 x 86 x 81 (Centimeters)	220 (Lbs) 100 (Kg)
Live Cell (Environmental Control)	21 x 34 x 19 (Inches) 53 x 86 x 48 (Centimeters)	210 (Lbs) 95 (Kg)

Related Collateral

• イメージング総合カタログ

• NEW ImageXpress Micro 4 High-Content Imaging System

• High Content Imaging solutions brochure
• MetaXpress Software brochure
• 3D Image Analysis Module Flyer

• 共焦点画像によるスフェロイドのハイスループットスクリーニング

• Evaluating cell cycle inhibitors using a live cell assay
• Assessment of neurotoxicity and neuronal development using induced pluripotent stem cell-based neurite outgrowth assay
• Phenotypic characterization of toxic compound effects on cardiomyocytes derived from induced pluripotent stem cells
• Apoptosis detection using EarlyTox Caspase-3/7-D NucView 488 Assay Kit on ImageXpress Micro systems
• Increase sensitivity no-wash assays using confocal imaging
• High-throughput imaging assays using zebrafish, a model organism for human disease
• Monitoring BNP expression and cell size
• Nuclear Translocation Screening Assay for ß-catenin modulators
• Multiplexed high content hepatotoxicity assays using iPSC-derived hepatocytes
• Intellligent time-lapse imaging to enhance live cell assay development
• High-content screening of neuronal toxicity using iPSC-derived human neurons
• Increase accuracy and efficiency of cytotoxicity assessment using the EarlyTox Live/Dead Assay Kit and high-content imaging
• High-throughput screening of single-cell contractile force