CloneSelect Imager ラベルフリー細胞増殖解析

細胞増殖の定量的評価と単クローン性の判定

CloneSelect™ Imagerは、白色透過光によるイメージングで細胞の増殖速度を客観的に定量し、コンフルエンシーを素早く測定します。マイクロプレートのウェル数に関係なく、プレート1枚の測定時間は90秒未満です。プレートにフタをしたまま測定できるので、測定後もインキュベーターに戻し培養を継続できます。細胞増殖は、すべてのプレートおよびウェルで観察され、追跡されるため、単クローン性の判定をサポートします。
  • Overview
  • Features
  • Applications
  • Models
  • Specifications
  • Resources

概要

  • 客観的かつ定量的に細胞増殖を評価
  • コロニー形成の過程を追跡
  • 簡単なソフトウェアで迅速に画像取得
  • イメージングから解析、レポートまでをサポート
  • 付着細胞や沈降させた浮遊細胞に最適
CloneSelect Imagerは、CHO細胞（DG44およびCHO-K1）、HEK細胞、ミエローマやハイブリドーマ、SupT1、Jurkat、H-4-II-E、MCF-7、PER.C6®、HT29、DLD1、KB31などの細胞株で利用されています。

アプリケーション

    • コンフルエンシーの測定
    • 細胞増殖の測定
    • 細胞数の推定
    • 細胞毒性の評価
    • 単クローン性（monoclonality）判定のサポート
    • コロニー形成試験
    • Cell migration assay

細胞密度を短時間に測定

同一ウェルの細胞増殖過程を簡単にトラッキング

細胞増殖曲線はソフトウェアにより自動的に作成され、同一のプレートを測定するたびに更新表示されます。また、ウェル内の様子をビジュアルに確認できるため、細胞の増殖過程を簡単にトラッキングできます。 データは測定と同時に自動保存されます。プレゼンテーション、データ検証、書類提出の際に役立ちます。

単クローン性の判定をサポート



Monoclonality Report 機能

手軽な操作で当局への申請用レポートを作成できます。ウェル全体の画像はもちろん、コロニー周辺領域の拡大画像やコロニー形成過程の画像をまとめてひとつのドキュメントにすることができます。

ハイスループットスクリーニング

  • 電子データのトラッキングで、ハイスループットプロセスを確実に管理
  • 蓋付きプレートを最大75プレートまで1度に処理可能
  • モレキュラーデバイスからの推奨と供給－CloneSelect Imagerに最適化済み
  • ハイスループットなラボにおける稼働中のCloneSelect Imagerをご覧ください

HIGH RESOLUTION ENABLE ACCURATE CELL DETECTION

Detect cells accurately even at challenging areas such as well edges

Single cell near well edge

Doublet near well edge

FLUORESCENCE IMAGING PROVIDES GREATER CERTAINTY

The fluorescence imaging capability adds an additional level of confidence as researchers use an optional fluorescence viability stain to track their cells. The software can automatically perform cell count analysis on the fluorescence images, making it easy to identify wells that contain a single cell.

AUTOMATED, RAPID CONFLUENCE DETERMINATION OF CELLS GROWING IN MICROPLATES

  • Intelligent software analysis automatically calculates confluence for each imaging time point
  • Shown here is the confluence percentage of cells growing in each well of a 96-well microplate.

AUTOMATICALLY PLOTS TIME COURSE OF CELL GROWTH

  • Microplate images are acquired over time
  • Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable

EASILY DOCUMENT MONOCLONALITY WITH A FEW CLICKS

With the ease of a few clicks, the Monoclonality Report feature organizes objective, quantitative, and qualitative data in an easily shareable report, enabling the presentation of image evidence needed to assure a high probability of clonality.

Example images from monoclonality report

  • Easily identify and select single-cell and artifact regions to include in report
  • Export high-resolution images of single cells, artifacts, and entire well (optional)
  • Automatically identify single cells with fluorescence version of CloneSelect Imager
  • Publish monoclonality report in PDF or Word Format

AUTOMATION COMPATIBLE

The CloneSelect Imager can be integrated with various 3rd party robotics to achieve high throughput

Example: Automated delivery of colony plates from an incubator to CloneSelect Imager and back
Instrument Setup:
1.CloneSelect Imager
2.Robotic arm
3.Incubator

Benefits:
  • Free up manual labor—customer can enjoy a walk-away time of 2-3 weeks
  • Reduce contamination and variation from plate handling
Click here to see more automation case studies with the CloneSelect Imager >>

CloneSelect Imager Application Suite

Cell Line Development Drug Discovery

PROOF OF CLONALITY

One of the regulatory requirements for biologics approval is to provide evidence that cell lines producing biotherapeutic proteins are derived from a single cell. The CloneSelect Imager can help in the following ways:
1. Save time and effort by reviewing wells that contain only a single colony with the intelligent “Loci of growth” function.
Instead of inspecting every single well in the plate, the “Loci of growth” function in the CloneSelect Imager software pinpoints wells that contain only 1 single colony, so users can save time and effort by only reviewing those wells.



2. Growth (image) history of each well can be tracked back to its starting point—providing evidence of monoclonality.

MONITOR COLONY OUTGROWTH

After screening and selecting colonies of interest, the CloneSelect Imager can monitor and evaluate subsequent colony outgrowth and productivity of the selected cell lines.


Outgrowth of a single well after 7 days – confluence overlay viewed for each imaging timepoint



The CloneSelect Imager can also estimate the number of cells per well. Data is then exported to enable normalization of results from ELISA or cell-based assays against cell number.



Correlate protein productivity and growth of cell line

COLONY FORMING ASSAY

Objective, Time-Saving Analysis
  • Every well can be imaged at any time point
  • Colony number and area quantified objectively
  • Focus on wells of interest, e.g. where growth is inhibited by test compounds

Review Results and Export Data
  • Dual gating for colony area and compactness
  • View each well at different time points
  • Export colony data



Save time by analyzing selected wells (highlighted in green).
Red wells are excluded.


View number of colonies per well


Colonies are identified in well images

ASSESSMENT OF CELL VIABILITY (MTT ASSAY)

CloneSelect Imager offers several benefits over traditional MTT assays for assessing cell viability in response to a cytotoxic challenge.
  • Replace time-consuming conventional techniques
  • Monitor cell death over time
  • Ensure a permanent record of data
  • Avoid costly reagents – no staining required

“THIS NEW IMAGE-BASED TECHNIQUE HAS THE POTENTIAL TO REPLACE THE CUMBERSOME MTT ASSAY WHEN FAST, UNBIASED AND HIGH-THROUGHPUT CYTOTOXICITY ASSAYS ARE REQUESTED.”

ACCURATE NON-INVASIVE IMAGE-BASED CYTOTOXICITY ASSAYS FOR CULTURED CELLS MARQUES-GALLEGO ET AL., BMC BIOTECHNOLOGY 2010, 10:43



Images of HeLa cells – wells exposed to different level of cytotoxin


Dose response curve: decreasing cell confluence with increasing exposure to cytotoxin


High resolution images show growth of cells exposed to low, medium and high cytotoxin levels

CELL MIGRATION ASSAY

CloneSelect Imager offers rapid, objective quantification of cell migration assays.
  • Determine migration rates, maximum migration rate and total migration area
  • Time-saving – scan a 96-well plate in <2 minutes
  • Convenient, label-free quantification

Review Results and Export Data
  • Obtain graphical display of migration area vs. time
  • Easy-to-read numerical and graphical output


Export data to determine
dose response to cell
mobility effectors


Wound healing time course

We offer 3 different models to suit your needs and budget.
  CloneSelect Imager Standard CloneSelect Imager HR CloneSelect Imager FL
Illumination
  • White light only
  • White light only
  • White light only
  • Fluorescence (up to 7 customizable fluorescence wavelengths available)
Pixel Resolution
  • 3.6 micron
  • 1.8 micron
  • 1.8 micron
Imaging Speed
  • < 90 sec for a 96-well microplate (white light only)
  • < 90 sec for a 96-well standard mode
  • < 8 minutes for a 96-well plate in high resolution mode (white light only)
  • < 10 min for a 96-well microplate (white light and fluorescence)
Autofocus Yes Yes Yes
Internal Barcode Reader Yes Yes Yes
Automation Compatible? Yes Yes Yes
*Download the specifications in the CloneSelect Imager brochure here.

Imaging
Software Dedicated imaging software pre-installed on a high-specification PC, Microsoft Windows 7
White light imaging Trans-illumination
Fluorescence imaging Optional (please contact us for details)
Data tracking 1 x internal barcode reader for data tracking of each plate
Camera High-resolution CCD camera
Imaging speed <90 sec in standard mode; <8 min in high resolution mode
Objective 4x
Resolution Standard: 3.6 micron; Maximum: 1.8 micron
Instrumentation
Source plate type Range of 6-, 12-, 24-, 96- and 384-well SBS microplates
Source plate capacity 1 x plates
Instrument dimensions 720 mm (width) x 428 mm (height) x 575 mm (depth)
Instrument weight 45 kg
Instrumentation
Source plate type Range of 6-, 12-, 24-, 96- and 384-well SBS microplates
Source plate capacity 1 x plates
Instrument dimensions 720 mm (width) x 428 mm (height) x 575 mm (depth)
Instrument weight 45 kg
regulATORY APPROVAL
Compliance CE
Quality ISO9001:2008 certified
AUTOMATION COMPATIBILITY
API suite available for robotic integration. Please contact us for details. 

Related Videos


Non-subjective interpretation of colony formation with the CloneSelect Imager

Related Collateral

Brochure
Applications
Posters

Flyer



Related Webinars


Identification and Selection of GPCR Cell Lines with ClonePix 2 System


Related Publications



Related Presentations


Developing High Throughput Mammalian Expression Platforms