CloneSelect Imager

無染色イメージングによる細胞増殖の客観的かつ定量的評価

CloneSelect™ Imagerは、高品質なイメージングとインテリジェントな画像解析により、時間のかかる主観的な手作業による検査を、一貫性のある客観的なものに変えます。細胞増殖は、すべてのプレートおよびウェルで観察され、追跡されます。

Overview
Features
Applications
Models
Specifications
Resources

概要

一貫性のある細胞密度の測定と細胞数の推定
増殖曲線の作成
生細胞の無染色白色光イメージング
付着細胞や沈降させた浮遊細胞に最適

CloneSelect Imagerは、CHO細胞（DG44およびCHO-K1）、HEK細胞、ミエローマやハイブリドーマ、SupT1、Jurkat、H-4-II-E、MCF-7、PER.C6®、HT29、DLD1、KB31などの細胞株で利用されています。

FACSや限界希釈法、あるいはClonePixによる細胞選別の後の細胞密度および細胞数の追跡

ハイスループットスクリーニング

電子データのトラッキングで、ハイスループットプロセスを確実に管理
蓋付きプレートを最大75プレートまで1度に処理可能
モレキュラーデバイスからの推奨と供給－CloneSelect Imagerに最適化済み
ハイスループットなラボにおける稼働中のCloneSelect Imagerをご覧ください

HIGH RESOLUTION ENABLE ACCURATE CELL DETECTION

Detect cells accurately even at challenging areas such as well edges

Single cell near well edge

Doublet near well edge

FLUORESCENCE IMAGING PROVIDES GREATER CERTAINTY

The fluorescence imaging capability adds an additional level of confidence as researchers use an optional fluorescence viability stain to track their cells. The software can automatically perform cell count analysis on the fluorescence images, making it easy to identify wells that contain a single cell.

AUTOMATED, RAPID CONFLUENCE DETERMINATION OF CELLS GROWING IN MICROPLATES

Intelligent software analysis automatically calculates confluence for each imaging time point
Shown here is the confluence percentage of cells growing in each well of a 96-well microplate.

AUTOMATICALLY PLOTS TIME COURSE OF CELL GROWTH

Microplate images are acquired over time
Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable

EASILY DOCUMENT MONOCLONALITY WITH A FEW CLICKS

With the ease of a few clicks, the Monoclonality Report feature organizes objective, quantitative, and qualitative data in an easily shareable report, enabling the presentation of image evidence needed to assure a high probability of clonality.

Example images from monoclonality report

Easily identify and select single-cell and artifact regions to include in report
Export high-resolution images of single cells, artifacts, and entire well (optional)
Automatically identify single cells with fluorescence version of CloneSelect Imager
Publish monoclonality report in PDF or Word Format

AUTOMATION COMPATIBLE

The CloneSelect Imager can be integrated with various 3rd party robotics to achieve high throughput

Example: Automated delivery of colony plates from an incubator to CloneSelect Imager and back

Instrument Setup:
1.CloneSelect Imager
2.Robotic arm
3.Incubator

Benefits:

Free up manual labor—customer can enjoy a walk-away time of 2-3 weeks
Reduce contamination and variation from plate handling

Click here to see more automation case studies with the CloneSelect Imager >>

CloneSelect Imager Application Suite

Cell Line Development

Proof of Clonality
Monitor Colony Outgrowth

Drug Discovery

Colony Forming Assays
Assessment of Cell Viability (MTT Assays)
Cell Migration Assays

PROOF OF CLONALITY

One of the regulatory requirements for biologics approval is to provide evidence that cell lines producing biotherapeutic proteins are derived from a single cell. The CloneSelect Imager can help in the following ways:
1. Save time and effort by reviewing wells that contain only a single colony with the intelligent “Loci of growth” function.
Instead of inspecting every single well in the plate, the “Loci of growth” function in the CloneSelect Imager software pinpoints wells that contain only 1 single colony, so users can save time and effort by only reviewing those wells.

2. Growth (image) history of each well can be tracked back to its starting point—providing evidence of monoclonality.

MONITOR COLONY OUTGROWTH

After screening and selecting colonies of interest, the CloneSelect Imager can monitor and evaluate subsequent colony outgrowth and productivity of the selected cell lines.

Outgrowth of a single well after 7 days – confluence overlay viewed for each imaging timepoint

The CloneSelect Imager can also estimate the number of cells per well. Data is then exported to enable normalization of results from ELISA or cell-based assays against cell number.

Correlate protein productivity and growth of cell line

COLONY FORMING ASSAY

Objective, Time-Saving Analysis

Every well can be imaged at any time point
Colony number and area quantified objectively
Focus on wells of interest, e.g. where growth is inhibited by test compounds

Review Results and Export Data

Dual gating for colony area and compactness
View each well at different time points
Export colony data

Save time by analyzing selected wells (highlighted in green).
Red wells are excluded.

View number of colonies per well

Colonies are identified in well images

ASSESSMENT OF CELL VIABILITY (MTT ASSAY)

CloneSelect Imager offers several benefits over traditional MTT assays for assessing cell viability in response to a cytotoxic challenge.

Replace time-consuming conventional techniques
Monitor cell death over time
Ensure a permanent record of data
Avoid costly reagents – no staining required

“THIS NEW IMAGE-BASED TECHNIQUE HAS THE POTENTIAL TO REPLACE THE CUMBERSOME MTT ASSAY WHEN FAST, UNBIASED AND HIGH-THROUGHPUT CYTOTOXICITY ASSAYS ARE REQUESTED.”

ACCURATE NON-INVASIVE IMAGE-BASED CYTOTOXICITY ASSAYS FOR CULTURED CELLS MARQUES-GALLEGO ET AL., BMC BIOTECHNOLOGY 2010, 10:43

Images of HeLa cells – wells exposed to different level of cytotoxin

Dose response curve: decreasing cell confluence with increasing exposure to cytotoxin

High resolution images show growth of cells exposed to low, medium and high cytotoxin levels

CELL MIGRATION ASSAY

CloneSelect Imager offers rapid, objective quantification of cell migration assays.

Determine migration rates, maximum migration rate and total migration area
Time-saving – scan a 96-well plate in <2 minutes
Convenient, label-free quantification

Review Results and Export Data

Obtain graphical display of migration area vs. time
Easy-to-read numerical and graphical output

Export data to determine
dose response to cell
mobility effectors

Wound healing time course

We offer 3 different models to suit your needs and budget.

	CloneSelect Imager Standard	CloneSelect Imager HR	CloneSelect Imager FL
Illumination	White light only	White light only	White light only Fluorescence (up to 7 customizable fluorescence wavelengths available)
Pixel Resolution	3.6 micron	1.8 micron	1.8 micron
Imaging Speed	< 90 sec for a 96-well microplate (white light only)	< 90 sec for a 96-well standard mode < 8 minutes for a 96-well plate in high resolution mode (white light only)	< 10 min for a 96-well microplate (white light and fluorescence)
Autofocus	Yes	Yes	Yes
Internal Barcode Reader	Yes	Yes	Yes
Automation Compatible?	Yes	Yes	Yes

*Download the specifications in the CloneSelect Imager brochure here.

Imaging
Software	Dedicated imaging software pre-installed on a high-specification PC, Microsoft Windows 7
White light imaging	Trans-illumination
Fluorescence imaging	Optional (please contact us for details)
Data tracking	1 x internal barcode reader for data tracking of each plate
Camera	High-resolution CCD camera
Imaging speed	<90 sec in standard mode; <8 min in high resolution mode
Objective	4x
Resolution	Standard: 3.6 micron; Maximum: 1.8 micron

Instrumentation
Source plate type	Range of 6-, 12-, 24-, 96- and 384-well SBS microplates
Source plate capacity	1 x plates
Instrument dimensions	720 mm (width) x 428 mm (height) x 575 mm (depth)
Instrument weight	45 kg

Instrumentation
Source plate type	Range of 6-, 12-, 24-, 96- and 384-well SBS microplates
Source plate capacity	1 x plates
Instrument dimensions	720 mm (width) x 428 mm (height) x 575 mm (depth)
Instrument weight	45 kg

regulATORY APPROVAL
Compliance	CE
Quality	ISO9001:2008 certified

AUTOMATION COMPATIBILITY
API suite available for robotic integration. Please contact us for details.