
細胞増殖の定量的評価と単クローン性の判定
CloneSelect™ Imagerは、白色透過光によるイメージングで細胞の増殖速度を客観的に定量し、コンフルエンシーを素早く測定します。マイクロプレートのウェル数に関係なく、プレート1枚の測定時間は90秒未満です。プレートにフタをしたまま測定できるので、測定後もインキュベーターに戻し培養を継続できます。細胞増殖は、すべてのプレートおよびウェルで観察され、追跡されるため、単クローン性の判定をサポートします。- Overview
- Features
- Applications
- Models
- Specifications
- Resources
概要
- 客観的かつ定量的に細胞増殖を評価
- コロニー形成の過程を追跡
- 簡単なソフトウェアで迅速に画像取得
- イメージングから解析、レポートまでをサポート
- 付着細胞や沈降させた浮遊細胞に最適
アプリケーション
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- コンフルエンシーの測定
- 細胞増殖の測定
- 細胞数の推定
- 細胞毒性の評価
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- 単クローン性(monoclonality)判定のサポート
- コロニー形成試験
- Cell migration assay
細胞密度を短時間に測定

同一ウェルの細胞増殖過程を簡単にトラッキング


単クローン性の判定をサポート

Monoclonality Report 機能
手軽な操作で当局への申請用レポートを作成できます。ウェル全体の画像はもちろん、コロニー周辺領域の拡大画像やコロニー形成過程の画像をまとめてひとつのドキュメントにすることができます。
ハイスループットスクリーニング
- 電子データのトラッキングで、ハイスループットプロセスを確実に管理
- 蓋付きプレートを最大75プレートまで1度に処理可能
- モレキュラーデバイスからの推奨と供給-CloneSelect Imagerに最適化済み
- ハイスループットなラボにおける稼働中のCloneSelect Imagerをご覧ください
HIGH RESOLUTION ENABLE ACCURATE CELL DETECTION
Detect cells accurately even at challenging areas such as well edges
Single cell near well edge

Doublet near well edge
FLUORESCENCE IMAGING PROVIDES GREATER CERTAINTY
The fluorescence imaging capability adds an additional level of confidence as researchers use an optional fluorescence viability stain to track their cells. The software can automatically perform cell count analysis on the fluorescence images, making it easy to identify wells that contain a single cell.
AUTOMATED, RAPID CONFLUENCE DETERMINATION OF CELLS GROWING IN MICROPLATES
- Intelligent software analysis automatically calculates confluence for each imaging time point
- Shown here is the confluence percentage of cells growing in each well of a 96-well microplate.

AUTOMATICALLY PLOTS TIME COURSE OF CELL GROWTH
- Microplate images are acquired over time
- Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable

EASILY DOCUMENT MONOCLONALITY WITH A FEW CLICKS
With the ease of a few clicks, the Monoclonality Report feature organizes objective, quantitative, and qualitative data in an easily shareable report, enabling the presentation of image evidence needed to assure a high probability of clonality.
Example images from monoclonality report
- Easily identify and select single-cell and artifact regions to include in report
- Export high-resolution images of single cells, artifacts, and entire well (optional)
- Automatically identify single cells with fluorescence version of CloneSelect Imager
- Publish monoclonality report in PDF or Word Format
AUTOMATION COMPATIBLE
The CloneSelect Imager can be integrated with various 3rd party robotics to achieve high throughputExample: Automated delivery of colony plates from an incubator to CloneSelect Imager and back

1.CloneSelect Imager
2.Robotic arm
3.Incubator
Benefits:
- Free up manual labor—customer can enjoy a walk-away time of 2-3 weeks
- Reduce contamination and variation from plate handling

CloneSelect Imager Application Suite
Cell Line Development Drug DiscoveryPROOF OF CLONALITY
One of the regulatory requirements for biologics approval is to provide evidence that cell lines producing biotherapeutic proteins are derived from a single cell. The CloneSelect Imager can help in the following ways:1. Save time and effort by reviewing wells that contain only a single colony with the intelligent “Loci of growth” function.
Instead of inspecting every single well in the plate, the “Loci of growth” function in the CloneSelect Imager software pinpoints wells that contain only 1 single colony, so users can save time and effort by only reviewing those wells.

2. Growth (image) history of each well can be tracked back to its starting point—providing evidence of monoclonality.

MONITOR COLONY OUTGROWTH
After screening and selecting colonies of interest, the CloneSelect Imager can monitor and evaluate subsequent colony outgrowth and productivity of the selected cell lines.
Outgrowth of a single well after 7 days – confluence overlay viewed for each imaging timepoint
The CloneSelect Imager can also estimate the number of cells per well. Data is then exported to enable normalization of results from ELISA or cell-based assays against cell number.


Correlate protein productivity and growth of cell line
COLONY FORMING ASSAY
- Every well can be imaged at any time point
- Colony number and area quantified objectively
- Focus on wells of interest, e.g. where growth is inhibited by test compounds
Review Results and Export Data
- Dual gating for colony area and compactness
- View each well at different time points
- Export colony data

Save time by analyzing selected wells (highlighted in green).
Red wells are excluded.

View number of colonies per well

Colonies are identified in well images
ASSESSMENT OF CELL VIABILITY (MTT ASSAY)
CloneSelect Imager offers several benefits over traditional MTT assays for assessing cell viability in response to a cytotoxic challenge.- Replace time-consuming conventional techniques
- Monitor cell death over time
- Ensure a permanent record of data
- Avoid costly reagents – no staining required
“THIS NEW IMAGE-BASED TECHNIQUE HAS THE POTENTIAL TO REPLACE THE CUMBERSOME MTT ASSAY WHEN FAST, UNBIASED AND HIGH-THROUGHPUT CYTOTOXICITY ASSAYS ARE REQUESTED.”
ACCURATE NON-INVASIVE IMAGE-BASED CYTOTOXICITY ASSAYS FOR CULTURED CELLS MARQUES-GALLEGO ET AL., BMC BIOTECHNOLOGY 2010, 10:43

Images of HeLa cells – wells exposed to different level of cytotoxin

Dose response curve: decreasing cell confluence with increasing exposure to cytotoxin

High resolution images show growth of cells exposed to low, medium and high cytotoxin levels
CELL MIGRATION ASSAY
CloneSelect Imager offers rapid, objective quantification of cell migration assays.- Determine migration rates, maximum migration rate and total migration area
- Time-saving – scan a 96-well plate in <2 minutes
- Convenient, label-free quantification
Review Results and Export Data
- Obtain graphical display of migration area vs. time
- Easy-to-read numerical and graphical output

Export data to determine
dose response to cell
mobility effectors

Wound healing time course
CloneSelect Imager Standard | CloneSelect Imager HR | CloneSelect Imager FL | |
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Illumination |
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Pixel Resolution |
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Imaging Speed |
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Autofocus | Yes | Yes | Yes |
Internal Barcode Reader | Yes | Yes | Yes |
Automation Compatible? | Yes | Yes | Yes |
Imaging | |
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Software | Dedicated imaging software pre-installed on a high-specification PC, Microsoft Windows 7 |
White light imaging | Trans-illumination |
Fluorescence imaging | Optional (please contact us for details) |
Data tracking | 1 x internal barcode reader for data tracking of each plate |
Camera | High-resolution CCD camera |
Imaging speed | <90 sec in standard mode; <8 min in high resolution mode |
Objective | 4x |
Resolution | Standard: 3.6 micron; Maximum: 1.8 micron |
Instrumentation | |
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Source plate type | Range of 6-, 12-, 24-, 96- and 384-well SBS microplates |
Source plate capacity | 1 x plates |
Instrument dimensions | 720 mm (width) x 428 mm (height) x 575 mm (depth) |
Instrument weight | 45 kg |
Instrumentation | |
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Source plate type | Range of 6-, 12-, 24-, 96- and 384-well SBS microplates |
Source plate capacity | 1 x plates |
Instrument dimensions | 720 mm (width) x 428 mm (height) x 575 mm (depth) |
Instrument weight | 45 kg |
regulATORY APPROVAL | ||
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Compliance | CE | |
Quality | ISO9001:2008 certified |
AUTOMATION COMPATIBILITY | |
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API suite available for robotic integration. Please contact us for details. |
Related Videos
Non-subjective interpretation of colony formation with the CloneSelect Imager
Related Collateral
BrochureApplications
- Improved productivity of cell line development using fully integrated automated solutions: a series of case studies with the CloneSelect Imager
- Enhanced Development of Virus-Specific Hybridomas using ClonePix and CloneSelect Imager Technologies
- Confluence and Growth Curve Generation
- Objective Quantification of Cell Migration
Posters
- Rapid Monoclonality Verification Methods to Boost Cell Line Development
- Non-Invasive, Label-Free Analysis of Cell Migration using CloneSelect Imager
- Imaging, Screening and Automated Selection of Mouse Embryonic Stem Cells
- Rapid Development of a Serum-Free, Suspension-Adapted CHO Cell Production Process
Flyer
Related Webinars
Identification and Selection of GPCR Cell Lines with ClonePix 2 System
Related Publications
- Accurate non-invasive image-based cytotoxicity assays for cultured cells.
Marqués-Gallego P, den Dulk H, Backendorf C, Brouwer J, Reedijk J, Burke JF.
BMC Biotechnol. 2010 Jun 17;10:43. doi: 10.1186/1472-6750-10-43. - Rapid Cell Line Development with Integrated Protein Analysis Clarke H.
Bioprocess International. 2010 Jul; 8:7.
Related Presentations
Developing High Throughput Mammalian Expression Platforms