
Gentle, thorough washing for your valuable microplates
Don’t let your transformative science be undone by incomplete or over-aggressive microplate washing. You can program your AquaMax® Microplate Washer with just the right plate or cell washing protocol for a thorough, gentle wash that wastes no time. Cell wash heads for 96- and 384-well formats have angled dispensing pins for extremely gentle washing of weakly adherent cell types. Integrate your AquaMax Washer with a StakMax Microplate Handling Stacker for batches up to 50 plates. AquaMax washers are compatible with all major microplate automation systems, too, for even more options.- Overview
- Applications
- Software
- Specifications
- Resources
User-Changeable Washer Heads
- 96- and 384-well Plate Wash Heads for biochemical assays for thorough washing of ELISAs
- 96- and 384-well Cell Wash Heads for cell-based assays with angled dispensing pins for gentle washing of cell-based assays
- Thirty second head changes with no tools, calibration, or alignment. Simply move a lever to engage and disengage.
- Buy the heads you need now and add heads as your needs change
- Simultaneous washing of all wells for 96- and 384-well plates minimizes processing time
Compatibility with Cell-Based Assays
HEK293 cells were seeded at 10,000 cells/well and incubated overnight. The cells were then labeled with Calcein AM dye. Cells were prewashed to remove most of the dye using the AquaMax 4000 Washer with the 96 Well Cell Wash Head. Cells were washed 2 times with 200 µL of wash buffer containing 1X PBS and at 5 mm above plate bottom. Cells were imaged using an ImageXpress Velos Laser-Scanning Cytometer. Cells were washed again 3 times with 300 µL of wash buffer at 3 mm above plate bottom. Cells were then imaged again to measure post-wash cell area. 95% of cells were retained in wells after washing.
- Cell-Wash Heads process plate gently enough for weakly adherent cell types (ex. HEK293)
- Angled dispensing pins and controllable dispensing pressure minimizes cell blow off
- Variable aspiration rate minimizes cell lift-off
- AquaMax Microplate Washer with Cell-Wash Head minimizes need for researchers to fix cells
Multiple Washer Reservoir Options
- Two- or four-fluid inlets with matching color-coded tubing facilitate quick assembly of the buffer and wash solution bottles and allow multiple wash protocols to be run without switching bottles
- 4 or 10 liter bottles available to address your throughput needs
- Level sensing bottles as an option to track liquid levels in the bottles
- Waste can be sent to reservoir or sink
Automatic Maintenance Protocols
- One-Touch Autoclean completely washes system with AquaMax Sterilant; rinses with water and then air dry both the aspiration and dispensing channel to prevent contaminant build up.
- Auto-shutdown is user-definable and can automatically rinse and purge AquaMax Washer with air, providing walk away flexibility at the end of the batch.
Automation Options
- Zero-alignment integration of AquaMax Microplate Washer to StakMax Microplate Handling Stacker provides easy automation option for most users.
- AquaMax onboard software controls the StakMax Microplate Handling Stacker
- AquaMax Microplate Washer can be integrated in the majority of automation environments, connects via USB port, and uses simple serial commands.
Any assay that needs washing as a primary, intermediary, or final step before quantitation is amenable to be washed on the AquaMax Microplate Washer. Two of the most widely used assays include:
- Biochemical assays, such as ELISA
- Cell-based assays, including weakly adherent cells
- High throughput screening (HTS) calcium mobilization and ion channel assays performed on Fluorometric Imaging Plate Readers including Molecular Devices FLIPR Tetra System and FlexStation 3 Multi-Mode Microplate Reader
- High content screening assays performed on imaging systems like Molecular Devices family of cellular imaging systems
Performance Specifications |
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Precision (96-well)
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< 4% CV @ 300 µL
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Precision (384-well)
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≤ 3% CV @ 100 µL
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Washing times 96 wash head, 300 µL/well
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Single cycle: 11 sec.
Three cycles: 26 sec.
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Washing times 384 wash head, 100 µL/well
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Single cycle: 12 sec.
Three cycles: 28 sec.
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Dispense volume
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10-400 µL per well
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FastWash times (optimized for speed)
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96 Wash Head, 300 µL/well, three cycles: 14 sec.
384 Wash Head, 100 µL/well, three cycles 16 sec.
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Process time with integrated, optional StakMax® Microplate Handling System (Fast Wash, 96 Wash Head, 300 µL/well, three cycles)
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< 13 min. for 20 plates
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Wash volume
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100-3000 µL
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Residual volume, 96
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≤ 5 µL
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Residual volume, 384
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≤ 2 µL
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General Specifications |
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Dimensions (in.)
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8.3 (H) x 18.3 (W) x 20.3 (D)
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Dimensions (cm)
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21 (H) x 46.6 (W) x 51.7 (D)
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Weight (instrument)
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40.8 lbs. (18.5 kg)
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Power supply
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Auto-switching
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Wash head
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PET (polyethylene terephthalate), 304 stainless steel
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Liquid contact materials
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Nylon, Polypropylene, Teflon, Polyethylene, Stainless Steel, and Silicone
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Compatible microplates
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SBS-conforming 96- and 384-well; flat, U-, or V-bottom plates
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Liquid inlets
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2 or 4
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Outlets
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Waste to drain or vented container
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Interface connections
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USB and proprietary StakMax connect
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Shake options
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3 speeds
Up to 600 sec. max. |
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Regulatory certifications
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cTUVus mark
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Related Video
Related Collateral
Brochure- AquaMax Microplate Washer
Applications
- Maximizing Cell Wash Performance with AquaMax 2000/4000 Microplate Washers and Cell Wash Heads
- Washing MAGPIX Beads with the AquaMax 2000/4000 Microplate Washer and LifeSep Magnetic Plate Holder
Posters
- FP and TR-FRET Kinase Assays with Automated Liquid Handling
- IMAP Kinase and Phosphatase Assays in Automated High Throughput Applications