AquaMaxMicroplate Washer

Gentle, thorough washing for your valuable microplates

Don’t let your transformative science be undone by incomplete or over-aggressive microplate washing. You can program your AquaMax® Microplate Washer with just the right plate or cell washing protocol for a thorough, gentle wash that wastes no time. Cell wash heads for 96- and 384-well formats have angled dispensing pins for extremely gentle washing of weakly adherent cell types. Integrate your AquaMax Washer with a StakMax Microplate Handling Stacker for batches up to 50 plates. AquaMax washers are compatible with all major microplate automation systems, too, for even more options.
  • Overview
  • Applications
  • Software
  • Specifications
  • Resources

User-Changeable Washer Heads

  • 96- and 384-well Plate Wash Heads for biochemical assays for thorough washing of ELISAs
  • 96- and 384-well Cell Wash Heads for cell-based assays with angled dispensing pins for gentle washing of cell-based assays
  • Thirty second head changes with no tools, calibration, or alignment. Simply move a lever to engage and disengage.
  • Buy the heads you need now and add heads as your needs change
  • Simultaneous washing of all wells for 96- and 384-well plates minimizes processing time

Compatibility with Cell-Based Assays

HEK293 cells were seeded at 10,000 cells/well and incubated overnight. The cells were then labeled with Calcein AM dye. Cells were prewashed to remove most of the dye using the AquaMax 4000 Washer with the 96 Well Cell Wash Head. Cells were washed 2 times with 200 µL of wash buffer containing 1X PBS and at 5 mm above plate bottom. Cells were imaged using an ImageXpress Velos Laser-Scanning Cytometer. Cells were washed again 3 times with 300 µL of wash buffer at 3 mm above plate bottom. Cells were then imaged again to measure post-wash cell area. 95% of cells were retained in wells after washing.
  • Cell-Wash Heads process plate gently enough for weakly adherent cell types (ex. HEK293)
  • Angled dispensing pins and controllable dispensing pressure minimizes cell blow off
  • Variable aspiration rate minimizes cell lift-off
  • AquaMax Microplate Washer with Cell-Wash Head minimizes need for researchers to fix cells

Multiple Washer Reservoir Options

  • Two- or four-fluid inlets with matching color-coded tubing facilitate quick assembly of the buffer and wash solution bottles and allow multiple wash protocols to be run without switching bottles
  • 4 or 10 liter bottles available to address your throughput needs
  • Level sensing bottles as an option to track liquid levels in the bottles
  • Waste can be sent to reservoir or sink

Automatic Maintenance Protocols

  • One-Touch Autoclean completely washes system with AquaMax Sterilant; rinses with water and then air dry both the aspiration and dispensing channel to prevent contaminant build up.
  • Auto-shutdown is user-definable and can automatically rinse and purge AquaMax Washer with air, providing walk away flexibility at the end of the batch.

Automation Options

  • Zero-alignment integration of AquaMax Microplate Washer to StakMax Microplate Handling Stacker provides easy automation option for most users.
  • AquaMax onboard software controls the StakMax Microplate Handling Stacker
  • AquaMax Microplate Washer can be integrated in the majority of automation environments, connects via USB port, and uses simple serial commands.
Any assay that needs washing as a primary, intermediary, or final step before quantitation is amenable to be washed on the AquaMax Microplate Washer. Two of the most widely used assays include:
  • Biochemical assays, such as ELISA
  • Cell-based assays, including weakly adherent cells
Although any assay where washing of microplates is needed would be appropriate and would benefit from automated washing on the AquaMax Washer. Examples include:
  • High throughput screening (HTS) calcium mobilization and ion channel assays performed on Fluorometric Imaging Plate Readers including Molecular Devices FLIPR Tetra System and FlexStation 3 Multi-Mode Microplate Reader
  • High content screening assays performed on imaging systems like Molecular Devices family of cellular imaging systems
The AquaMax Microplate Washer has an intuitive touch-screen user interface that includes:
  • Up to 100 different programs, each of which contains up to 50 program steps
  • Configurable to wash plates and perform system maintenance
  • Various system head designs and control features optimize the ability to run biochemical and cell-based microplate washing applications
  • Precise dispensing and aspiration control that allows both fast processing of robust biochemical assays and gentle processing of cell-based assays without cell disruption
  • Fast sequential washing, continuous washing, and bottom washing options that enable the AquaMax Washer to be set up to meet specific assay needs while reducing the wash time to a minimum

Performance Specifications

Precision (96-well)
< 4% CV @ 300 µL
Precision (384-well)
≤ 3% CV @ 100 µL
Washing times 96 wash head, 300 µL/well
Single cycle:  11 sec.
Three cycles:  26 sec.
Washing times 384 wash head, 100 µL/well
Single cycle:  12 sec.
Three cycles:  28 sec.
Dispense volume
10-400 µL per well
FastWash times (optimized for speed)
96 Wash Head, 300 µL/well, three cycles: 14 sec.
384 Wash Head, 100 µL/well, three cycles 16 sec.
Process time with integrated, optional StakMax® Microplate Handling System (Fast Wash, 96 Wash Head, 300 µL/well, three cycles)
< 13 min. for 20 plates
Wash volume
100-3000 µL
Residual volume, 96
≤ 5 µL
Residual volume, 384
≤ 2 µL

General Specifications

Dimensions (in.)
8.3 (H) x 18.3 (W) x 20.3 (D)
Dimensions (cm)
21 (H) x 46.6 (W) x 51.7 (D)
Weight (instrument)
40.8 lbs. (18.5 kg)
Power supply
Wash head
PET (polyethylene terephthalate), 304 stainless steel
Liquid contact materials
Nylon, Polypropylene, Teflon, Polyethylene, Stainless Steel, and Silicone
Compatible microplates
SBS-conforming 96- and 384-well; flat, U-, or V-bottom plates
Liquid inlets
2 or 4
Waste to drain or vented container
Interface connections
USB and proprietary StakMax connect
Shake options
3 speeds
Up to 600 sec. max.
Regulatory certifications
cTUVus mark

Related Video

See the AquaMax Microplate Washer in action in this short video.

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