Stemonix uses three of our solutions
to validate their microBrain 3D Assay Ready Plates
The FLIPR® Tetra® High-Throughput Cellular Screening System provides an automated solution for identifying early leads in the drug discovery process and for evaluating drug efficacy and toxicity. With simultaneous pipette and read function, the system supports fast kinetic cellular assays. It can be quickly configured based on library size, detection mode, screening format, assay and target. As a fully integrated solution, the transition from assay development to lead optimization is seamless.
The ImageXpress® Micro Confocal High-Content Imaging System helps you expand the boundaries of your research with the ultimate combination of speed, sensitivity and flexibility. Capture high quality images of whole organism, thick tissues, 3D spheroid assays, and cellular or intracellular events. Combined with MetaXpress® High-Content Image Acquisition and Analysis Software, the ImageXpress Micro Confocal system provides a complete multi-dimensional, high-throughput screening solution to help you discover your next landmark scientific breakthrough.
The SpectraMax i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system.
The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. Using the ImageXpress Micro Confocal system for brightfield capture and automated size measurement, highly homogenous size distribution with typical coefficients of variation of less than 4% across an individual plate have been observed. Immunofluorescence analysis using the ImageXpress microscope revealed that these 3D spheroids are composed of cortical neurons and astrocytes that display key markers of cellular maturity, such as synaptic proteins. Moreover, these spheroids presented spontaneous and synchronized calcium oscillations that are easily detected on both the FLIPR and ImageXpress systems. The functional maturity of the microBrain 3D platform was confirmed by examining a panel of neuromodulators with known mechanisms of action. Using the FLIPR system, modulation of neural activity was detected that correlated with the expected activity of these compounds, and confirmed that the spheroids possess functional glutamatergic and GABAergic circuits.
Finally, a targeted library of compounds with different potencies against the Zika Virus infection was tested in a toxicological case study. First, the SpectraMax® i3x Multi-Mode Microplate Reader (Molecular Devices) was employed to investigate cell toxicity of this compound library on the microBrain 3D platform. The cellular toxicity analysis was then complemented with functional toxicity, investigated in a high throughput fashion with the FLIPR system. Lastly, high-resolution videos of calcium oscillations following each treatment were generated using the ImageXpress confocal microscope. Altogether, integration of microBrain 3D in multiple platforms resulted in extensive characterization of the toxicological profile of a targeted library and demonstrated the feasibility of integrating this platform for in vitro investigations of complex neural phenotypes, toxicological profiles, and drug screening.
StemoniX microBrain 3D Assay Ready Plates as a Human iPSC-based Neural Spheroid Platform for High Throughput Drug Screening