M. Yves Cambet is a researcher within the Readers, Assay Development & Screening (RE.A.D.S) Unit at the Faculty of Medicine in the Centre Medical Univerisitaire (C.M.U.) of Geneva, Switzerland. The main objectives of the RE.A.D.S Unit are to provide expertise in assay development and screening to all scientists within the faculty. The group support a wide range of assays, from enzymatic target to cell-based assays, in both 96- and 384- well formats.
To support this vital work, researchers use a variety of state-of-the-art microplate readers, including:
- The FlexStation® 3 Multi-Mode Microplate Reader for ion channel assays such as membrane potential or calcium assays
- The SpectraMax® L Microplate Reader for all luciferase-based assays
- The SpectraMax® Paradigm Multi-Mode Microplate Reader for a wide range of other assays such as ELISA, cytotoxicity, Bradford, cell viability assays, etc.
Two of these readers are equipped with a system of injection which allows scientists to measure several wells simultaneously in kinetic mode.
By analysing all their data in the SoftMax® Pro Data Acquisition and Analysis Software, Yves is able to provide support for the calculation of pharmacological parameters such as IC50, Emax, Km, Kd, etc.