The organ-on-a-chip company
MIMETAS offers the OrganoPlate®, a unique 3D organ-on-a-chip platform. The OrganoPlate® is a fully compatible microfluidic culture plate, enabling testing of compounds in any throughput on miniaturized organ models. The OrganoPlate® supports 3D cell culture under continuous perfusion, membrane-free co-culture, and boundary and gradient formation, thus mimicking important aspects of tissues and organs, translating into better predictive tissue and disease models. Based on the OrganoPlate®, MIMETAS develops custom tissue models for the pharmaceutical industry to speed up and improve screening and development of new medicines and personalized therapies. An overview of the models in the OrganoPlate® can be seen in a recent webinar, sponsored by Molecular Devices.
At MIMETAS, they make use of the OrganoPlate® to develop disease, toxicology, and transport models for research, development and drug screening. A variety of tissue models has been developed, including brain models, kidney toxicity models, cancer models, liver models, gut models, and endothelial vasculature models. These vasculature models can be combined with other tissue cultures, to establish vascularized tissue models.
For development and application of tissue models, they use a range of image-based readouts, such as phase-contrast time lapse, fluorescence microscopy, confocal microscopy, in combination with automated 3D image analysis. The team use the ImageXpress Micro Confocal High-Content Imaging System on a daily basis, serving as their standard high-throughput imager for most in-chip assays.
Jos Joore, Managing Director and Co-Founder of Mimetas, says, “The ImageXpress Micro Confocal enables us to use our 3D organ-on-a-chip platform as a true high-throughput system. Both the ImageXpress Micro and the ImageXpress Micro Confocal are a perfect match for the OrganoPlate®.”
Microfluidic Kidney Proximal Tubule Model in the MIMETAS OrganoPlate®.
Human kidney cells (Sigma RPTEC) were seeded against Collagen 1 and stained for Ezrin (red) and Dapi (blue).
Ezrin represents the microvilli on apical side of proximal tubule.
Wevers, N.R. et al. High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform. Nature Scientific Reports 6, Article number: 38856 (2016).
Junaida A. et al. An End-User Perspective on Organ-on-a-Chip: Assays and Usability Aspects. Current Opinion in Biomedical Engineering. Article in Press (2017).