At the Dana-Farber Cancer Institute, the Center for Cancer Systems Biology (CCSB) utilizes experimental and computational strategies to uncover complex biological interactions. Using a wealth of technology platforms and bioinformatics tools, they have generated protein-protein interaction (PPI), or “interactome”, maps for a variety of organisms. High-quality binary PPIs are generated using variants of yeast two-hybrid methodologies combined with validation using orthogonal in vivo and in vitro approaches. Unbiased experimental data can be integrated with literature research and computational analysis to aid their discoveries.
The Molecular Devices QPix™ 460 provides this experimental ability by screening and picking microbial libraries in a high-throughput manner. The QPix helps them increase both the speed as well as volume at which protein-protein interactions which are discovered, enabling them to rapidly expand the high-quality interactome maps.