Capture 96- and 384-well microplate absorbance data that fuels your imagination
Be inspired by answers to an infinite range of research questions with the SpectraMax® 340PC384 Microplate Reader, our tunable visible absorbance reader for 96- and 384-well microplates. Acquire data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Our SpectraMax® 340PC384 Microplate Reader comes with temperature control up to 45 °C, a robotics-compatible interface, and SoftMax® Pro Data Analysis Software. PathCheck® Sensor technology automatically measures the liquid depth of each well, and can also be used to calculate concentrations without a standard curve and expand the dynamic range of your readings to 4.0 OD. Our SpectraMax 340PC384 Microplate Reader will run so many of your applications, including ELISA, cytoxicity, ß- galatosidase reporter gene assays, and NAD(P)H-based assays.- Overview
- Applications
- Software
- Specifications
- Resources
Benefits
- No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 340-850 nm in 1 nm increments
- Easy assay optimization: Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine wavelength peaks
- Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck Technology, the only temperature-independent pathlength correction method
- Thorough mixing of samples in microplate wells with user-programmable Automix feature
- Comprehensive data analysis with SoftMax® Pro Software
- Seamless integration with the StakMax® Microplate Handling Stacker
Microplate Reader Validation
- Comprehensive validation toolset available include:
- SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes,
- SoftMax Pro Software Validation Package
- Software tools for FDA 21 CFR Part 11 compliance
The SpectraMax 340PC384 Microplate Reader supports a multitude of absorbance assay categories including:
- Cell viability, proliferation, and cytotoxicity
- ELISAs and immunoassays
- Endotoxin detection
- Enzyme assays
- Microbial growth
- Phosphotases/kinases
- Protein quantitation
- Reporter gene assays
SoftMax Pro Software simplifies setting up your assays by providing:
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- Simple set-up and automatic instrument recognition
- Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
- A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
- Standard data reduction settings are preselected as default
- Automatic data recovery feature
SoftMax Pro Software offers a variety of customizing options:
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- Predefined calculation options for common data analysis functions
- Automatic export to Microsoft Excel
- Instrument settings and plate read area can be adjusted to suit your experiment’s needs
- Custom data reduction settings can be selected for optimal signal selection
- Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience
- Contemporary user community enabling protocol exchange and expertise sharing among our numerous users
- Proven integration with leading automation and LIMS platforms for partnered solutions
- Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
* available in select instrument models
| General Specifications | |
| Dimensions (in) | 8.6 (h) x 22.8 (w) x 15 (d) |
| Dimensions (cm) | 22 (h) x 58 (w) x 38 (d) |
| Weight | 30 lb (13.6 kg) |
| Power source | 90-250 VAC, 50/60 Hz |
| Performance Specifications | |
| Wavelength Range | 340 – 850 nm |
| Wavelength Selection | Monochromator, tunable 1.0 nm increments |
| Wavelength Bandwidth | ≤ 2.0 nm |
| Wavelength Accuracy | ± 1.0 nm |
| Wavelength Repeatability | ± 0.2 nm |
| Photometric Range | 0.0 to 4.0 OD |
| Photometric Resolution | 0.001 OD |
| Photometric Accuracy | < ± 0.006 OD ± 1.0%, 0.0 – 2.0 OD |
| Photometric Precision | < ± 0.003 OD ± 1.0%, 0.0 – 2.0 OD |
| Stray Light | ≤ 0.07% at 340 nm OD ≥ 3.15 |
| Light Source | Xenon flash lamp |
| Microplate Read Time – Endpoint | 96-well: 9 seconds 384-well: 29 seconds |
| Microplate Read Time – Kinetic | 96-well: 9 second interval 384-well: 29 second interval |
| Temperature Range | Ambient + 4 °C up to 45 °C |
| Temperature Uniformity | ± 0.5 °C @ 37 °C |
Related Collateral
Data SheetSpectraMax 340PC384 Microplate Reader
Brochures
Applications
- Optical density measurements automatically corrected to a 1-cm pathlength with PathCheck Technology
- DNA and RNA absorbance measurements using SpectraMax Microplate Readers
- High-throughput melamine detection with Abraxis Melamine ELISA Kit and Molecular Devices Absorbance readers
- High-throughput melamine detection with Beacon Analytical Systems Melamine Plate Kit and Molecular Devices Absorbance readers
- High-throughput melamine detection with Romer Labs AgraQuant Melamine Assays and Molecular Devices Absorbance readers