SpectraMax Plus 384Microplate Reader

Begin your brilliant projects quickly, and then go even faster, with rapid UV-Vis absorbance reads for a variety of sample formats

Get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. The SpectraMax® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on the same instrument. Monochromator wavelength selection saves you the time and trouble of ever having to change out filters. Typical applications for this powerful reader include DNA and RNA quantification, enzyme kinetic assays, and ELISA based assays. When your project is ready for high throughput, take advantage of StakMax® automated microplate handling compatibility for full robotic integration.

  • Overview
  • Applications
  • Software
  • Specifications
  • Resources

Benefits

  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 190-1000 nm in 1 nm increments
  • Easy assay optimization: Read a variety of sample formats from cuvettes to 96- and 384-well microplates in endpoint, kinetic, and spectral scan modes
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy.
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature Quickly quantify DNA, RNA, and protein samples as low as 2 µL with the SpectraDrop Micro-Volume Microplate
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker

Microplate Reader Validation

  • Comprehensive validation toolset available include:
    • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes,
    • SoftMax Pro Software Validation Package
    • Software tools for FDA 21 CFR Part 11 compliance
The SpectraMax Plus384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Micro volume applications
  • Microbial growth
  • Nucleic acid (DNA) quantitation
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays

SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature




SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment’s needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience
  • Contemporary user community enabling protocol exchange and expertise sharing among our numerous users


  • Proven integration with leading automation and LIMS platforms for partnered solutions
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software

* available in select instrument models

General Specifications
Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 30 lbs (13.6 kg)
Power Source 90-250 VAC, 50/60 Hz
Performance Specifications
Wavelength Range 190-1000nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth ≤ 2.0 nm
Wavelength Accuracy < ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy (Microplate) < ± 0.006 OD ± 1.0%, 0.0 – 2.0 OD
Photometric Accuracy (Cuvette) < ± 0.005 OD ± 1.0%, 0.0 – 2.0 OD
Photometric Precision ± 0.003 OD ± 1.0%, 0.0 – 2.0 OD
Stray Light ≤ 0.07% at 230 nm
OD ≥ 3.15
Light Source Xenon Flash Lamp
Microplate Read Time – Endpoint 96-Well: 9 seconds
384-Well: 29 Seconds
Microplate Read Time – Kinetic 96-Well: 9 second interval
384-Well: 29 second internal
Microplate Read Time – Speed Read Mode 96-Well: 5 seconds
384-Well: 16 seconds
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity (Microplate) ± 0.5 °C @ 37 °C