SpectraMax 340PC384Microplate Reader

Capture 96- and 384-well microplate absorbance data that fuels your imagination

Be inspired by answers to an infinite range of research questions with the SpectraMax® 340PC384 Microplate Reader, our tunable visible absorbance reader for 96- and 384-well microplates. Acquire data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. Our SpectraMax® 340PC384 Microplate Reader comes with temperature control up to 45 °C, a robotics-compatible interface, and SoftMax® Pro Data Analysis Software. PathCheck® Sensor technology automatically measures the liquid depth of each well, and can also be used to calculate concentrations without a standard curve and expand the dynamic range of your readings to 4.0 OD. Our SpectraMax 340PC384 Microplate Reader will run so many of your applications, including ELISA, cytoxicity, ß- galatosidase reporter gene assays, and NAD(P)H-based assays.

  • Overview
  • Applications
  • Software
  • Specifications
  • Resources

Benefits

  • No filter system: Includes monochromator allowing up to 6 wavelengths at a time in the visible wavelength range and scanning ranges from 340-850 nm in 1 nm increments
  • Easy assay optimization: Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine wavelength peaks
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells with user-programmable Automix feature
  • Comprehensive data analysis with SoftMax® Pro Software
  • Seamless integration with the StakMax® Microplate Handling Stacker

Microplate Reader Validation

  • Comprehensive validation toolset available include:
    • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes,
    • SoftMax Pro Software Validation Package
    • Software tools for FDA 21 CFR Part 11 compliance
The SpectraMax 340PC384 Microplate Reader supports a multitude of absorbance assay categories including:

  • Cell viability, proliferation, and cytotoxicity
  • ELISAs and immunoassays
  • Endotoxin detection
  • Enzyme assays
  • Microbial growth
  • Phosphotases/kinases
  • Protein quantitation
  • Reporter gene assays

SoftMax Pro Software simplifies setting up your assays by providing:

  • Simple set-up and automatic instrument recognition
  • Pre-defined protocols for endpoint and kinetic ELISA protein plus quantification assays such as Bradford, Lowry, BCA, and DC protein assays
  • A wide variety of scan types: Endpoint, Kinetic, Spectrum* and Well Scanning*
  • Standard data reduction settings are preselected as default
  • Automatic data recovery feature




SoftMax Pro Software offers a variety of customizing options:

  • Predefined calculation options for common data analysis functions
  • Automatic export to Microsoft Excel
  • Instrument settings and plate read area can be adjusted to suit your experiment’s needs
  • Custom data reduction settings can be selected for optimal signal selection
  • Full reports, with font flexibility, and live mini-tables and graphs are now available within the Notes section to simplify the result sharing experience
  • Contemporary user community enabling protocol exchange and expertise sharing among our numerous users


  • Proven integration with leading automation and LIMS platforms for partnered solutions
  • Optional tools for regulated labs with SoftMax Pro GxP Compliance Software

* available in select instrument models

General Specifications
Dimensions (in) 8.6 (h) x 22.8 (w) x 15 (d)
Dimensions (cm) 22 (h) x 58 (w) x 38 (d)
Weight 30 lb (13.6 kg)
Power source 90-250 VAC, 50/60 Hz
Performance Specifications
Wavelength Range 340 – 850 nm
Wavelength Selection Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth ≤ 2.0 nm
Wavelength Accuracy ± 1.0 nm
Wavelength Repeatability ± 0.2 nm
Photometric Range 0.0 to 4.0 OD
Photometric Resolution 0.001 OD
Photometric Accuracy < ± 0.006 OD ± 1.0%, 0.0 – 2.0 OD
Photometric Precision < ± 0.003 OD ± 1.0%, 0.0 – 2.0 OD
Stray Light ≤ 0.07% at 340 nm
OD ≥ 3.15
Light Source Xenon flash lamp
Microplate Read Time – Endpoint 96-well: 9 seconds
384-well: 29 seconds
Microplate Read Time – Kinetic 96-well: 9 second interval
384-well: 29 second interval
Temperature Range Ambient + 4 °C up to 45 °C
Temperature Uniformity ± 0.5 °C @ 37 °C