QPix400 Imager全自動微生物コロニーピッキングシステム

モレキュラーデバイスの全自動微生物コロニーピッキングシステムでは、マーカーを事前にスクリーニングし、希望するコロニーだけをピッキングできます。

QPix™ 400シリーズの微生物コロニーピッカーは、ピッキング前のスクリーニングの段階でコロニーの検出と蛍光マーカーの定量を同時に実施する独自のオプションを提供してます。モレキュラーデバイスのQPixシステムは、世界中の研究機関、シークエンシング施設、バイオテック企業、製薬会社などで600台以上が導入されています。QPixシステムのロボティクスは、ヒトゲノムプロジェクトが推進されていた当時、シークエンシング施設において信頼性と正確さで有名になりました。

  • Overview
  • Applications
  • Compatibility
  • specifications
  • Resources
  • Citations

コロニーピッカー以上の機能として、QPixシステムにより、以下のことが可能となります。

  • サンプリング、播種からピッキングまでの自動ワークフロー
  • 毎回正しいコロニーをピッキング
  • タンパク質発現、バイオ燃料研究、酵素進化、ファージディスプレイ、DNAシークエンシング、ライブラリーの作成と管理のために、大規模で多様なクローン集団を効率的に管理
時間を短縮、不要な作業を軽減、経費を削減
様々なアプリケーションをサポート
比類のない性能
ワークフローおよびアプリケーションの要件に合わせてシステムをカスタマイズ
比色コロニースクリーニング – 青白色選定
阻止円の検出
プラークのピッキング
タンパク質工学、タンパク質進化、指向性進化または酵素進化
タンパク質発現、形質転換およびサブクローンの管理
合成生物学、バイオ燃料研究および酵素進化
ファージディスプレイ
DNAシークエンシング
クローンの管理 – メタゲノミクス
クローンの管理 – ライブラリスクリーニング

Refer to the following pages for a list of compatible reagents and supplies

*Download the specifications in our QPix System brochure here.

 QPIX SYSTEM SPECIFICATIONS
IMAGING 
Camera (white light only system) CCD Camera, image resolution: 22 pixels /mm. Field of view: 62 x 46 mm
Camera (white light and fluorescence system) CCD Camera, image resolution 22 pixels /mm. Field of view: 32 x 24 mm
White light imaging Trans-illumination
Fluorescent imaging (optional) Epifluorescence illumination, five standard wavelengths included: 
Ex /Em: 377 /447 nm for DAPI /Hoechst 
Ex /Em: 457 /536 nm for FITC /GFP 
Ex /Em: 531 /593 nm for Cy3 /DS Red 
Ex /Em: 628 /692 nm for Cy5 
Ex /Em: 531 /624 nm for Rhodamine /Texas Red
Colony statistics Colonies selectable based on size, proximity, roundness. Selection performed on whole tray image
Tracking 1 x barcode reader for tracking of source and destination plates. Data tracked through all applications for plates with same I.D.
SOFTWARE
Plate replication Software license and additional head
Re-arraying Software license and additional head
Gridding Software license and additional head
Zone of inhibition detection Software license
Colorimetric colony selection Software license and filters
Low contrast colony detection (plaque picking)  Included with Software 2.0 and newer
INSTRUMENTATION
Spreading capability (QPix 460 System only) Sampling and spreading source plate: 1 x 96-well (30 minutes for 96 samples). Aspiration volume 10-130 µL. Destination plate: 2 x 22 cm 48 region QTrays. Samples spread from pre-set or customizable pattern
Destination plate capacity • QPix 420 System: Picking: 12 plates; replicating and re-arraying, maximum of 20 plate positions
• QPix 450 System: Up to 210 low profile plates, 70 per stacker lane, maximum 3 stackers
• QPix 460 System: Up to 140 low profile plates, 70 per stacker lane, maximum 2 stackers
Source plate capacity • QPix 420 System: Without manual intervention: 1 x 15 cm petri dish; 5 x 9 cm petri dishes; 2 x OmniTrays; 1 x 22 cm QTrays
• QPix 450/460 System: Without manual intervention: 2 x 15 cm petri dish; 10 x 9 cm petri dishes; 4 x OmniTrays; 2 x 22 cm QTrays
Picking destination plate type Various, 24-, 48-, 96-, or 384-well, including deep well
Picking height Integrated ultrasonic agar height sensor to set agar height per plate for accurate picking
Picking head Fully pneumatic, 96 pin picking head. Interchangeable heads for other applications
Picking pin types Range of organism-specific pins
Picking capacity 3000 colonies per hour in white light, 2000 colonies per hour in fluorescent light
Fluorescent picking Colonies imaged in white light for location identification and fluorescence for data analysis. WL and FL image multiplexed.
Fluorescence data Multiple parameters available e.g. interior mean. Fluorescent intensity recorded for picked colonies.
Wash bath 3 x static wash baths
Pin drying Proprietary halogen pin drying station
Dimensions  • QPix 420 System (without table): 1460 mm (width) x 770 mm (depth) x 750 mm (height)
• QPix 450/460 System: 2200 mm (width, excluding monitor arm) x 800 mm (depth) x 2140 mm (height on table)
Compressed air specifications
Air Clean, oil-free with sub-micron filtration
Minimum operating pressure 6 bar (~90psi)
Minimum operating volume 80L/min
Optional compressor specifications
Compressor unit Clean, oil-free compressor with sub-micron filtration
Dimensions 250 mm (width) x 600 mm (depth) x 750 mm (height)
Weight 60 kg
Minimum operating pressure 6 bar
Minimum operating volume 80 L/min
Noise level 61 dB(A)
Regulatory approval
Compliance CE 
Quality ISO9001:2008 certified

関連ビデオ

QPixシステムの高速オペレーションと正確なコロニーピッキング

Watch the QPix System’s high speed operation and accurate colony picking in action.

Watch the QPix System’s Overview Video

Related Webinars

  • Synthetic Metagenomics – Converting Digital Information Back to Biology

Related Publications

More Publications

Latest Citations:


For a complete list of citations, please click here.

 

Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome

EP Culligan, RD Sleator, JR Marchesi, C Hill – The ISME journal, 2012 – nature.com
 manufacturer’s instructions. A Genetix (Berkshire, UK) QPix 2 XT colony-picking robot
was used to transfer fosmid clones to 384-well micro-titre plates, which were stored at
−80 °C until needed. Screening the metagenomic library. A total 

The QPIX 400 systems: Next generation robotic microbial colony pickers with enhanced software and colony selection features

P Tawde – 36th Symposium on Biotechnology for Fuels and …, 2014 – sim.confex.com
The QPix 400 series of robotic microbial colony picking platforms are members of the 
Molecular Devices product line that provides state-of-the-art automated solutions for the 
genomics, proteomics, industrial microbiology and biofuels community. The entire 
 

Genetic Engineering & Biotechnology News

D BioSciences – online.liebertpub.com
 on display at SLAS. The 400 series, which includes the QPix 450 and QPix 460,
with a third system, the QPix 400, to follow later in 2012, features the option to
simultaneously detect colonies and quantify fluorescent markers. 
 

Impact of interspecific interactions on antimicrobial activity among soil bacteria

O Tyc, M van den Berg, S Gerards… – Frontiers in …, 2014 – ncbi.nlm.nih.gov
 Figure 1 Figure 1. Workflow of the high-throughput interaction assay. (A) Overview of the
antimicrobial screening: bacteria were inoculated with a Genetix Qpix 2 colony picking robot
either in monoculture or in one-to-one interactions on OmniTray™ plates. For the … 
 

Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacter sp. strain DSM 17874

M Throne-Holst, A Wentzel, TE Ellingsen… – Applied and …, 2007 – Am Soc Microbiol
 The plates were incubated at 30°C for about 48 h, and colonies were picked using a QPix
robot (Genetix) and transferred into 96-well microtiter plates (Nunc) containing 120 μl LB
supplemented with chloramphenicol and kanamycin in each well. 

High-throughput screening and selection of high lipid producers for biofuels

W Lew – 37th Symposium on Biotechnology for Fuels and …, 2015 – sim.confex.com
 This method can be easily generalized to other species and other indicators. We cultured
a high-lipid accumulating strain of R. opacus with Nile Red and used a QPix 420 to select
and pick colonies based on morphology and fluorescence intensity. 
 

A Functional Metagenomic Analysis of the Human Gut Microbiome to Identify Genes Involved in Osmotolerance

EP Culligan – 2008 – cmbn.no
 Methods: 1. NaCl growth curves were carried out to determine salt tolerance. 2. Over 20,000
clones were screened using QPix 2 XT robotic gridding machine. 3. Transposon mutagenesis
carried out using a hyperactive Tn5 in vitro transposition system (EZ-Tn5). Results: 
 

LIFE SCIENCE TECHNOLOGIES

EAC TEST – sciencepubs.com
 MICROBIAL COLONY PICKERS The QPix 400 series, the next generation of microbial colony
pickers, offers unmatched performance and productivity enabling scientists to manage large,
diverse populations, including 98% efficiency in colony picking, far surpassing the 
 
 

Construction and characterization of two bacterial artificial chromosome libraries of pea (Pisum sativum L.) for the isolation of economically important genes

F Belzile, CJ Coyne, MT McClendon, JG Walling… – …, 2007 – NRC Research Press
 containing LB, the appropriate antibiotic, and 10% (v/v) freezing medium (Zhang 2000) either
by hand using sterile wooden toothpicks (plates 1–45) or using a Flexsys GS2 robotic workstation
(plates 46–145; Genomic Solutions, Ann Arbor, Michigan) or a QPix robot (plates 146 
 
 

Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome

EP Culligan, RD Sleator, JR Marchesi, C Hill – 2013 – dx.plos.org
 The library was screened as outlined previously [21]. Briefly, a total of 23,040 clones from the
library were screened on LB agar supplemented with 6.5% (w/v) NaCl and 12.5μg/ml
chloramphenicol using a Genetix QPix 2 XT™ colony picking/gridding robotics platform.