Gemini EMMicroplate Reader

Re-invent your fluorescence assays

Create custom fluorescence assays without having to purchase expensive filter sets. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets you determine the optimal excitation and emission settings for your creative new fluorescence intensity assays. If the best wavelengths are unknown, the Gemini EM reader will find them for you. Switch easily between top and bottom reading modes for solution- and cell-based assays.

  • Overview
  • Applications
  • Software
  • Specifications
  • Resources

Benefits

  • Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
  • Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
  • Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
  • Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
  • Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well

Measuring Accurately and Reproducibly

  • Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
  • Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
  • Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices own integrated microplate stacker designed exclusively for the SpectraMax Reader

Comprehensive Reader Validation

Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:
  • SpectraTest ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
  • SoftMax Pro Software Validation Package
  • Software tools for FDA 21 CFR Part 11 compliance
The Gemini EM Microplate Reader supports a multitude of fluorescence assay categories including:

  • ELISAs and immunoassays
  • Nucleic acid (DNA) quantitation
  • Protein quantitation
  • Reporter gene assays
  • Cell Viability, proliferation, and cytotoxicity
  • Enzyme assays
  • Transporter assays
  • Phosphotases/kinases
  • Microbial growth
SoftMax® Pro Software is the most published microplate reader control and microplate data analysis software package available to scientists. The inclusive package of ready-to-run protocols, analysis algorithms, and 21 different curve fit options provides the full solution from data acquisition to analysis to publishing. The FDA 21 CFR Part 11 compliance tools available in SoftMax Pro GxP Software for regulated laboratories deliver a complete solution to minimize validation costs, provide end-to-end chain of custody, and eliminate the need to validate additional analysis software.
General Specifications
Dimensions (in) 8.6 H x 22.8 W x 15 D
Dimensions (cm) 22 H x 58 W x 38 D
Weight 35 lbs (16 kg)
Power Source 100-240 VAC, 50/60 Hz
Performance Specifications
Wavelength Range-Excitation 250-850 nm
Wavelength Range-Emission 250-850 nm
Wavelength Selections Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth (Excitation and emission) 9nm
Wavelength Accuracy < ± 2.0 nm
Top Read Sensitivity 3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity 8.0 fmol/well FITC 200 µl in 96 wells
Light Source Xenon flash lamp
Microplate Read Time
Normal Mode 96 wells 15 seconds
384 wells 45 seconds
Dynamic Range 6 orders of magnitude
Temperature Range Ambient + 4°C up to 45°C
Temperature Unitformity (Microplate) ± 1°C @ 37°C