Comparison of Microplate Readers with Luminescence Detection

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SpectraMax i3x
Multi-Mode
Detection Platform

SpectraMax Paradigm
Multi-Mode
Microplate Reader

SpectraMax M Series
Multi-Mode
Microplate Readers

FlexStation 3
Multi-Mode
Microplate Reader

SpectraMax L
Microplate Reader

FilterMax F3 & F5
Multi-Mode
Microplate Readers
Detector Photomultiplier tubes (PMT)
and/or photodiode
2 PMT Photo-multiplier tube 2 PMT 1, or 2 Ultra-fast
Photomultiplier Tubes
Silicon Photodiode + PMT
Spectral range 300-850 nm 300-850 nm 250-850 nm 250-850 nm 380-630 nm F3: 400 – 650nm
FilterMax F5: 400 – 750 nm
Dynamic range > 6 decades > 6 decades > 6 decades > 6 decades > 9 decades > 6 decades
Sensitivity 3 pM for 96-wells,
6 pM for 384-wells
(Optimized for ATP-Glow)
1.25 pM for 96-wells,
3 pM for 384-wells,
19 pM for 1536-wells
(Optimized for ATP-Glow)
43 pM ATP in 96 wells
(optimized)
< 2 fg/well for firefly luciferase in 96- and 384-well top read < 20 attomol ATP per well
Microplate format 6 to 1536 wells* 6 to 1536 wells 6-384-well microplates 6-384
(96- and 384-wells with pipetting)
96- and 384-well microplates F3: 96 – 384 well
F5: 6 – 1536 well
Cross-talk < 0.4% in white 96- and 384-well microplates < 0.3% in white 96- and 384-well microplates < 0.3% in white 96- and 384-well microplates < 3 x 10-5 (96-well)
Read modes Spectral, Endpoint, Kinetic Endpoint, Kinetic Endpoint, Kinetic Endpoint, Kinetic, Fast Kinetic Endpoint, Kinetic,
Fast Kinetic, Dual-read
Endpoint, Kinetic,
Linear Scan, Area Scan
Read times 0:30 minutes for 96-wells,
1:15 minutes for 384-wells
2:00 minutes for 96-wells,
7:00 minutes for 384-wells
2:00 minutes for 96-wells,
7:00 minutes for 384-wells
0.1 up to 3600 sec.
in steps of 0.1 sec.
18sec/96well
37sec/384well
Injectors/pipettors Option available Option available 8- or 16-channel pipettor 2 variable volume injectors
Injection/pipettor volumes Max. volume 200uL
for 8-channel and 30 uL
for 16-channel
Temperature control 2 degrees C above
ambient to 45 degrees C
2 degrees C above
ambient to 45 degrees C
2 degrees C above
ambient to 45 degrees C
2 degrees C above
ambient to 45 degrees C
5 degrees C above
ambient to 45 degrees C
Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software SoftMax Pro Software
Validation & compliance tools IQ/OQ Protocols,
SoftMax Pro GxP
Compliance Software,
SpectraTest LM1
Validation Plate
SoftMax Pro GxP
Compliance Software
IQ/OQ Protocols,
FDA 21 CFR
Part 11 Compliance,
SpectraTest LM1
Validation Plate
IQ/OQ/PQ Protocols,
FDA 21 CFR
Part 11 Compliance
Software Validation Package,
IQ/OQ Documentation,
Physical Validation Test Plate
IQ/OQ Protocols,
SoftMax Pro GxP
Compliance Software,
SpectraTest LM1
Validation Plate
Robotic compatible
Features



* 1536 detection available via detection cartridges