Comparison of Microplate Readers with Fluorescence Detection


SpectraMax i3x
Multi-Mode
Detection Platform

SpectraMax M Series
Multi-Mode
Microplate Readers

SpectraMax
Gemini EM
Microplate Reader

SpectraMax
Gemini XPS
Microplate Reader

FlexStation 3
Multi-Mode
Microplate Reader

FilterMax F3 & F5
Multi-Mode
Microplate Readers
Fluorescence
Absorbance
Luminescence +
Time-resolved fluorescence (TRF) + * * +
HTRF Certified +
Fluorescence Polarization + +
Wavelength range FL 250-850 nm,
Abs 30 – 1000 nm,
Lum 300-850 nm
FL 250 – 850,
Abs 200 – 1000
FL 250 – 850 ex,
250-850 em
FL 250 – 850 ex,
360 – 850 em
FL 250 – 850 ex,
250 – 850 em;
Abs 200 – 1000
FL 230 – 750 nm +
Abs 230 – 650 nm +
Bandwidth Adjustable:
9/15 nm (ex),
15/25 nm (em)
9/9 or 9/15+ 9/9 9/9 9/15
Tunable wavelength selection/ monochromator
Endpoint
Kinetic
Spectral scanning
Well scanning
Reading speed (sec) 15-17+ 15 15 17 18 seconds/96 well
37 seconds/384 well
Microplate formats 6 to 1536 wells° 6-384 6-384 6-384 6-384** FilterMax F3:
96 – 384 well
FilterMax F5:
6 – 1536 well
Integrated
multi-channel pipettor
***
Temperature control +
Top reading +
Bottom reading +
Cuvette port
Validation tools
Robotics /
automation
Features



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* Secondary modes
** 96- and 384-well with pipetting
*** 8- and 16-channel pipettor
+ Varies by model
° 1536 detection available via detection cartridges.