ClonePix 2 System

哺乳動物細胞のハイスループットスクリーニングおよび客観的な選別

  ClonePix™ 2システムでは、従来の技術と比べて、短時間でより多くのクローンを自動的にスクリーニングし、最適な発現レベルの細胞を選択し、正確にコロニーをピッキングします。ClonePixシステムは、現在、世界中の100ヶ所以上の研究所で利用されており、ワークフローの生産性を向上させることで、標的タンパク質の特性評価や新規プロジェクトにより多くの時間を割くことができるようになっています。多数のバイオファーマ企業が日常業務にClonePixシステムを取り入れており、科学的な出版物や会議においてデータがますます引用されています。
  • Overview
  • Applications
  • Technology
  • Specifications
  • Resources
  • Citations

概要

  • 評価の順位付けに従って、最適な発現レベルの細胞を選択します。最適なタンパク質産生細胞を見つける可能性を高めます。
  • 細胞株や抗体の開発期間を短縮させます – 限界希釈法によるクローン選別を避けられます。
  • in situでの発現レベルに基づき、タンパク質産生能が低い細胞を早期に除けます。
  • 正確かつ高い信頼度でコロニーをピッキングします。ロボティクスの再設計によってコロニーに問題が生じるリスクを低減します。

バイオ治療学のための抗体開発

最適な分泌細胞株の選定
バイオ治療学のための細胞株の開発
不安定なクローンの排除

研究用の抗体の探索

抗体開発のための高力価ハイブリドーマ細胞株の同定
抗原特異的ハイブリドーマまたはB細胞のスクリーニングおよび選別

タンパク質の発現および生産

GPCR発現細胞株の選別および開発
内在性レポーターの発現による選別
細胞表面発現マーカーによる選別
タグ付き組換えタンパク質分泌細胞の選別

幹細胞スクリーニング/幹細胞株の維持

コロニーの形態を利用した細胞株の維持
コロニー形成
検出法の選択
イメージングと解析
ランキングとデータ表示
CLONEPIX 2 SYSTEM SPECIFICATIONS
IMAGING 
Software Dedicated imaging software pre-installed on a high-specification PC, Microsoft Windows 7
White light imaging • Trans-illumination for imaging low contrast colonies such as adherent monolayers or small colonies in suspension
• Epi-illumination for imaging colonies as they are collected
Data tracking Internal barcode reader for source and destination plates enables data tracking for each run
Camera Integrated 16-bit cooled CCD camera
Imaging speed 6-well microplate: 5 min for 2 wavelengths (standard conditions)
Resolution Standard: 28 micron; Maximum: 1.5 micron
INSTRUMENTATION
Containment Fully enclosed working environment with Class 100-type, HEPA filtration
Source plate type PetriWell-6 plate, PetriWell-1 plate, Greiner 6 well plate, Nunc 6 well plate, Nunc OmniTray
Destination plate type PetriWell-96 plate, Costar 96 well plate, Greiner 96 well plate, Nunc 96 well plate, Falcon 96 well plate
Source plate capacity 10 plates
Destination plate capacity 10 plates
Picking head 8 x picking pins – each pin independently controlled
Picking pin size Diameter of picking pins is application specific – F1: suspension cells, F2: adherent cells
Picking speed > 200 clones per hour
Wash bath Ethanol wash bath, automatically refilled
Picking system fluids 5 L sterile water supply, 5L waste bottle
Pin drying Proprietary halogen pin drying station
Instrument dimensions 1010 mm (width) x 900 mm (depth) x 1490 mm (height)
Instrument weight 350 kg
COMPRESSED AIR SPECIFICATIONS
Air Clean, oil-free with sub-micron filtration
Minimum operating pressure 6 bar (~90psi)
Minimum operating volume 80 L/min
OPTIONAL COMPRESSOR SPECIFICATIONS
Compressor unit Clean, oil-free compressor with sub-micron filtration
Dimensions 250 mm (width) x 600 mm (depth) x 750 mm (height)
Weight 60 kg
Minimum operating pressure 6 bar
Minimum operating volume 80 L/min
Noise level 61 dB(A
REGULATORY APPROVAL
Compliance CE
Quality ISO9001:2008 certified

Related Webinars

  • Improved Hybridoma Discovery for Immunodiagnostics Using ClonePix and CloneSelect Imager Technologies
  • Identification and Selection of GPCR Cell Lines with ClonePix 2 System

Related Publications

More Publications

日本語版

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Latest Citations:

For a complete list of citations, please click here.

RELATED CITATIONS

High-throughput ClonePix FL analysis of mAb-expressing clones using the UCOE expression system

JJC Hou, BS Hughes, M Smede, KM Leung, K Levine… – New biotechnology, 2014 – Elsevier
Therapeutic recombinant monoclonal antibodies (mAbs) are commonly produced by high-expressing, clonal, mammalian cells. Creation of these clones for manufacturing remains heavily reliant on stringent selection and gene amplification, which in turn can lead to 

Rapid isolation of antigen-specific clones from hybridoma fusions

CJ Mann – Nature Methods, 2007 – nature.com
 The Genetix ClonePix FL is a powerful way to find and collect antigen-specific clones from hybridoma fusions in a rapid one-step process—shortening the process timeline and allowing parallel interrogation of multiple antigens in the monoclonal antibody discovery process. 

Stem and iPS cell selection: quantitation of surface marker (SSEA1) and intracellular GFP

CJ Mann, ENC Newman, DJ Whitney, NJ Latchem… – Nature …, 2007 – test.nature.com
 The Genetix ClonePix FL technology, already established for the rapid one-step identification and isolation of hybridomas and cell lines for biopharmaceutical production, can be used to isolate and collect stem cells expressing specific cell surface and intracellular proteins. 

Improving Clone Production for Increased Protein Yield from Mammalian Cell Lines

S Williams, R Cranenburgh – Innovations in Pharmaceutical …, 2008 – iptonline.com
 An alternative approach for the screening of secreting clones is the use of cell imaging systems such CellXpress marketed by Sigma-Aldrich Corporation, and ClonePix FL from Genetix Ltd. ClonePix FL can screen around ten thousand in three weeks. 

Rapid automated selection of mammalian cell colonies by cell surface protein expression

ENC Newman, D Whitney – Nature Methods| Application Notes, 2007 – nature.com
 Top of page Abstract. Genetix’s unique ClonePix FL technology uses our ability to grow mammalian cells into clonal colonies suspended in semisolid media, and image these colonies using fluorescence assays and markers. 

An improved clone selection method

U Jamnikar, M Blas, D Gaser… – Proceedings of the 21st …, 2012 – Springer
 In most cases there are no resources to isolate and analyze more than few 100 clones. ClonePix FL (CP-FL, Genetix) technology substantially increases the number of screened clones in the selected pool before expansion in microtiter plates.  1.2.1 ClonePix FL. 

Micropallet arrays for the separation of single, adherent cells

G To’a Salazar, Y Wang, G Young, M Bachman… – Analytical …, 2007 – ACS Publications
 the sample during collection. ClonePix (Genetix, Hampshire, UK) is an automated system that uses image recognition to guide a suction pipet that aspirates colonies of loosely adherent cells from plates. The system requires 

High‐Throughput Technologies in Bioprocess Development

T Carrier, E Heldin, M Ahnfelt, E Brekkan… – Encyclopedia of …, 2010 – Wiley Online Library
 Cell Line Development Technology Developer ClonePix Fl Genetix LEAPTM Cyntellect FACS Multiple developers  The ClonePix Fl technology, developed by Genetix, capitalizes on a system in which mammalian cells are cultured in a semisolid media and then imaged using 1 

Screening and Subcloning of High Producer Transfectomas Using Semisolid Media and Automated Colony Picker

S Dharshanan, CS Hung – Monoclonal Antibodies, 2014 – Springer
 3.2 Sanitization and Calibration of ClonePix FL System. Diligently follow the instructions of the pre-programmed software in the ClonePix FL system.  10. Sanitize the system with ultraviolet light for 10 min. 3.3 Selection of High-Producing Transfectomas Using ClonePix FL System. 

Screening and Selection of GPCR-Expressing Cell Lines

A Glaser – 2014 – online.liebertpub.com
 The ClonePix™ 2 System from Molecular Devices represents a proven, one-step method of screening large cell populations rapidly.  The ClonePix 2 System is a platform enabling the identification and isolation of GPCR clones in an accurate and rapid manner.