Patch-clamp amplifiers from single channels to large macroscopic recordings

The Axon Instruments® series of amplifiers provide best-in-class solutions for the entire range of patch-clamp experiments. The portfolio of amplifiers includes Axopatch™ 200B for ultra low-noise single-channel recordings, MultiClamp™ 700B for whole-cell voltage-clamp and high-speed current-clamp recordings, and Axoclamp™ 900A for two-electrode voltage-clamp and current-clamp recordings.
  • Eliminate filters

    Minimize signal to noise ratio

    The Axopatch 200B Capacitor Feedback Patch Clamp Amplifier offers one of the lowest-noise single-channel recordings available via innovative capacitor-feedback technology.
  • Get consistent results

    Perform multi-channel experiments

    The MultiClamp 700B Microelectrode Amplifier enables whole-cell voltage-clamp and current-clamp recordings. It is the most versatile amplifier in the portfolio.
  • Integrate with ease

    Measure large currents

    Large output compliance range of our Axoclamp 900A Microelectrode Amplifier facilitates the measurement of large and rapid voltage-clamp and current-clamp recordings.
SpectraMax ABS and ABS Plus Absorbance Microplate Readers
Using the Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels

Features

  • Actively cooled headstage

    The Axopatch 200B amplifier features proprietary technology that provides active headstage cooling that reduces electrical noise close to the theoretical limits of physics.
  • Software control of settings

    The MultiClamp 700B and Axoclamp 900A amplifiers offer software control. Software control streamlines setup, and enables automation of parameters, telegraphing, and advanced protocols.
  • Support up to four headstages

    The MultiClamp 700B supports up to two primary CV-7B headstages and two optional auxiliary headstages (HS-2 or VG-2 type) enabling multi-channel recording for cellular network studies.
  • Large output compliance range

    The Axoclamp 900A amplifier supports the measurement of larger currents and ensures faster clamp speed (±180 V in TEVC and HVIC modes).
  • Multiple modes of operation

    The Axoclamp 900A amplifier offers 5 modes of operation: current clamp, discontinuous current clamp, two-electrode voltage clamp, discontinuous single-electrode voltage clamp, high-voltage current clamp.
  • Works with any data acquisition system

    The family of amplifiers integrates with most data acquisition programs. The pCLAMP™ 11 Software and DigiData® 1550B system for data acquisition and analysis provide optimal performance.

Applications of Axon Instruments Patch-Clamp Amplifiers

  • ELISA

    Single Channel Recording

    The patch-clamp technique involves a glass micropipette forming a tight gigaohm seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. To measure single ion channels, a “patch” of membrane is pulled away from the cell after forming a gigaohm seal. If a single ion channel is within the patch, currents can be measured. The Axopatch 200B, with extremely low-noise profile, is ideal for this application, maximizing signal for the smallest conductance ion channels.
  • Ion Channels

    An ion channel is a group of proteins that form a pore across the lipid bilayer of a cell. Each channel is permeable to a specific ion (examples: potassium, sodium, calcium, chloride). Patch-clamp is used to evaluate current or voltage in the membrane associated with ion channel activity via direct measurement in real time using ultra-sensitive amplifiers, high-quality data acquisition systems, and powerful software to evaluate the results.
    ELISA
  • ELISA

    Patch Clamp

    The patch-clamp technique involves a glass micropipette forming a tight gigaohm (GΩ) seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. The whole-cell technique involves rupturing a patch of membrane with mild suction to provide low-resistance electrical access, allowing control of transmembrane voltage. Alternatively, investigators can pull a patch of membrane away from the cell and evaluate currents through single channels via the inside-out or outside-out patch-clamp technique.
  • Whole Cell Recording

    The whole cell patch-clamp technique involves a glass micropipette forming a tight gigaohm (GΩ) seal with the cell membrane. This micropipette contains a wire bathed in an electrolytic solution to conduct ions. A patch of membrane is subsequently ruptured by mild suction so that the glass micropipette provides a low-resistance access to the whole cell, thereby allowing the investigator to control the transmembrane voltage and allowing the investigator to evaluate the sum of all currents through membrane bound ion channels.
    ELISA
  • ELISA

    Series Resistance Compensation

    Series resistance is the sum of all resistances between the amplifier and the inside of the cell using the whole-cell recording method. Due to Ohms Law, the larger this resistance, the greater the difference between the command level and the measured values. This creates an error in actual voltage or current measurement potentially leading to inaccurate observations. To overcome this, the Molecular Devices amplifiers have built-in circuitry to improve the bandwidth of the recording by compensating the error introduced by the voltage or current drop across the series resistance.
  • Voltage Clamp Amplifier

    In an experiment using the voltage-clamp method, the investigator controls the membrane voltage in a cell and measures the transmembrane current required to maintain that voltage. This voltage control is called a command voltage. To maintain this command voltage level, an amplifier must inject current. The current injected will be equal and opposite the current escaping through open ion channels, allowing the amplifier to measure the amount of current passing through open membrane bound ion channels.
    ELISA
  • ELISA

    Current Clamp Amplifier

    Current-clamp is a method used to measure the resulting membrane potential (voltage) from an injection of current. To measure the membrane potential, the MultiClamp 700B and Axoclamp 900A both monitor voltage drop initiated by current injection along an in-series resistor. Current-clamp is commonly used to inject simulated, but realistic current waveforms into a cell, and monitor membrane effect. This technique is ideal for the evaluation of important cellular events such as action potentials.

Specifications & Options of Axon Instruments Patch-Clamp Amplifiers

Axopatch™ 200B Capacitor Feedback Patch Clamp Amplifier

MultiClamp™ 700B Microelectrode Amplifier

Axoclamp™ 900A Microelectrode Amplifier

General specifications
Applications

Single-channel recording, whole-cell patch-clamp recording, extracellular recording, amperometry/voltammetry, bilayer recording, nanopore study

Single-channel recording, whole-cell patch-clamp recording, intracelluar sharp electrode recording, extracellular recording, amperometry/voltammetry, bilayer recording, nanopore study

Whole-cell current-clamp recording, intracelluar sharp electrode recording, extracellular recording, two-electrode voltage-clamp recording

Smart telegraph

Gain, filter, capacitance

Gain, filter, capacitance, input/output scaling factors, recording mode – 2 channels

Gain, filter, capacitance, input/output scaling factors, recording mode – 2 channels

Seal test

Holding potential range

-/+ 1000 mV

-/+ 1000 mV

-/+ 200 mV for dSEVC and TEVC modes

Holding current range

-/+ 200 nA

-/+ 200 nA

Up to -/+ 1/Ro (feedback resistor)

Output gain (mV/pA)

0.5 to 500

1.0 to 2000

1.0 to 2000

Modes

I-Clamp, V-Clamp

I-Clamp, V-Clamp

I-Clamp, DCC, HVIC, dSEVC, TEVC

Open circuit noise in 5kHz and 10 kHz

0.06 pA rms, (5 kHz), 0.13 pA rms (10 kHz)

0.15 pA rms, (5 kHz), 0.28 pA rms (10 kHz)

True voltage clamp-resistor feedback

True for for cSEVC

True for for cSEVC

True for for dSEVC

Capacitance Compensation

RMS noise

0.13 pA rms (10 kHz) increases to only 0.145 pA RMS when a patch-pipette holder is attached

0.28 pA rms (10 kHz) at 50 GΩ to 3.0 pA rms (10 kHz) at 50 MΩ

20 mV at 10 kHz

Automatic oscillation suppression

Automated mode-switching between current clamp and voltage clamp mode

Slow current injection to maintain assigned potential

True current clamp-voltage follower

Simultaneous dual patch clamping recordings

Computer control*

Conventional interface

* Holding level, current passing, filter option, multiple signal outputs, pipette offset, fast and whole cell capacitance compensation, series compensation, pipette neutralization, bridge balance

Resources of Axon Instruments Patch-Clamp Amplifiers

Videos and Demos

Calculate Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
How to Combine Traces, Calculate Rise or Decay Time Constant, and Perform Curve Fitting Using Axon pCLAMP Software
Create Customized Command Waveforms Using pCLAMP Software
How to Create Customized Command Waveforms Using the pCLAMP Software
Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software
The Use of Sequencing Keys, User List, and Stimulus File with pCLAMP Software
Synchronizing Electrophysiology and Imaging Solutions with pCLAMP and MetaMorph Software
Synchronizing Electrophysiology and Imaging Solution with Axon pCLAMP and MetaMorph Software
Membrane Test Between Sweeps in Clampex & Analysis of Synaptic Events with Clampfit Data Analysis
Online Statistics, Membrane Test Between Sweeps in Clampex and Analysis of Synaptic Events with the Clampfit™ Data Analysis
Use of Axoporator for Single-cell Electroporation for Transfection and Dye-labeling
Use of the Axoporator 800A for Single-cell Electroporation for Transfection and Dye-labeling
Using Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels
Using the Axoclamp 900A for Two-Electrode Voltage-Clamp of Xenopus Oocytes Expressing Ion Channels
Use of Filters in Data Acquisition and the Clampfit Application
Writing Long-Term Potentiation and Depression Protocols and the Use of Filters in Data Acquisition and the Clampfit Application
Resistance Compensated Series
Series Resistance Compensated or Not
Using Electrophysiological Studies to Accelerate Mechanistic in Reception and Transmission
Using Electrophysiological Studies to Accelerate Mechanistic Study in Reception and Transmission
Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning
Update and Hardware Choices for Optogenetics Considerations for Synchronized Light Patterning
Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in Xenopus Oocyte Model
Investigations of the Effects of Amyloid-Beta Proteins on hSlo1.1, a BK Channel, in a Xenopus Oocyte Model
Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies
Nanopores-Electronic Tools for Single-Molecule Biophysics and Bio-Nanotechnologies
Axon Amplifiers and pCLAMP Software-key Features Reviews (Chinese version)
Axon Amplifiers and pCLAMP Software-key Features Reviews (Chinese version)
Using Clampfit in Basic Single Channel Analysis
Basic Single Channel Analysis Using Clampfit
Action Potential Analysis in Clampfit Module
Action Potential Analysis in Clampfit Module
A Walkthrough of Protocol Editor in pCLAMP Data Acquisition Module
A Walkthrough of the Protocol Editor in the pCLAMP Data Acquisition Module
A Walkthrough of Protocol Editor in pCLAMP (Chinese version)
A Walkthrough of Protocol Editor in pCLAMP (Chinese version)

Ready to get started?

We are ready to help you solve your tough research challenges. Our proven solutions and highly qualified teams across the globe can help advance your next big discovery.